Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis

The aim of this study was to estimate the therapeutic potential of specific egg yolk immunoglobulin (IgY) on dermatophytosis caused by Trichophyton rubrum. The IgY was produced by immunizing hens with cell wall proteins of T. rubrum, extracted from eggs by PEG precipitation and then purified by ammo...

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Autores principales: Xiao, Yire, Hu, Qingqing, Jiao, Luoying, Cui, Xiping, Wu, Panpan, He, Pan, Xia, Nana, Lv, Rui, Liang, Yuxin, Zhao, Suqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd. 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126877/
https://www.ncbi.nlm.nih.gov/pubmed/31513894
http://dx.doi.org/10.1016/j.micpath.2019.103741
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author Xiao, Yire
Hu, Qingqing
Jiao, Luoying
Cui, Xiping
Wu, Panpan
He, Pan
Xia, Nana
Lv, Rui
Liang, Yuxin
Zhao, Suqing
author_facet Xiao, Yire
Hu, Qingqing
Jiao, Luoying
Cui, Xiping
Wu, Panpan
He, Pan
Xia, Nana
Lv, Rui
Liang, Yuxin
Zhao, Suqing
author_sort Xiao, Yire
collection PubMed
description The aim of this study was to estimate the therapeutic potential of specific egg yolk immunoglobulin (IgY) on dermatophytosis caused by Trichophyton rubrum. The IgY was produced by immunizing hens with cell wall proteins of T. rubrum, extracted from eggs by PEG precipitation and then purified by ammonium sulfate precipitation. The cross-reactivity (CR) with other fungi, growth inhibition on T. rubrum in vitro and therapeutic effect on T. rubrum infection in BALB/C mice of the specific IgY were then evaluated. Anti- T. rubrum cell wall proteins IgY (anti-trCWP IgY) presented a certain degree of cross-reactivity with different fungi. In the in vitro and in vivo activity researches, Anti-trCWP IgY showed a significant dose-dependent growth inhibitory effect on T. rubrum in vitro and a significant dose-dependent therapeutic effect on T. rubrum infection in BALB/C mice.
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spelling pubmed-71268772020-04-08 Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis Xiao, Yire Hu, Qingqing Jiao, Luoying Cui, Xiping Wu, Panpan He, Pan Xia, Nana Lv, Rui Liang, Yuxin Zhao, Suqing Microb Pathog Article The aim of this study was to estimate the therapeutic potential of specific egg yolk immunoglobulin (IgY) on dermatophytosis caused by Trichophyton rubrum. The IgY was produced by immunizing hens with cell wall proteins of T. rubrum, extracted from eggs by PEG precipitation and then purified by ammonium sulfate precipitation. The cross-reactivity (CR) with other fungi, growth inhibition on T. rubrum in vitro and therapeutic effect on T. rubrum infection in BALB/C mice of the specific IgY were then evaluated. Anti- T. rubrum cell wall proteins IgY (anti-trCWP IgY) presented a certain degree of cross-reactivity with different fungi. In the in vitro and in vivo activity researches, Anti-trCWP IgY showed a significant dose-dependent growth inhibitory effect on T. rubrum in vitro and a significant dose-dependent therapeutic effect on T. rubrum infection in BALB/C mice. Published by Elsevier Ltd. 2019-12 2019-09-09 /pmc/articles/PMC7126877/ /pubmed/31513894 http://dx.doi.org/10.1016/j.micpath.2019.103741 Text en © 2019 Published by Elsevier Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Xiao, Yire
Hu, Qingqing
Jiao, Luoying
Cui, Xiping
Wu, Panpan
He, Pan
Xia, Nana
Lv, Rui
Liang, Yuxin
Zhao, Suqing
Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis
title Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis
title_full Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis
title_fullStr Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis
title_full_unstemmed Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis
title_short Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis
title_sort production of anti-trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126877/
https://www.ncbi.nlm.nih.gov/pubmed/31513894
http://dx.doi.org/10.1016/j.micpath.2019.103741
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