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Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease

The association of Mycoplasma cynos with canine infectious respiratory disease is increasingly being recognised. This study describes the strain typing of 14 M. cynos isolates cultured from trachea and bronchoalveolar lavage samples of six dogs with respiratory disease, from two separate kennels in...

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Autores principales: Mannering, Sally A., McAuliffe, Laura, Lawes, Joanna R., Erles, Kerstin, Brownlie, Joe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126931/
https://www.ncbi.nlm.nih.gov/pubmed/18977617
http://dx.doi.org/10.1016/j.vetmic.2008.09.058
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author Mannering, Sally A.
McAuliffe, Laura
Lawes, Joanna R.
Erles, Kerstin
Brownlie, Joe
author_facet Mannering, Sally A.
McAuliffe, Laura
Lawes, Joanna R.
Erles, Kerstin
Brownlie, Joe
author_sort Mannering, Sally A.
collection PubMed
description The association of Mycoplasma cynos with canine infectious respiratory disease is increasingly being recognised. This study describes the strain typing of 14 M. cynos isolates cultured from trachea and bronchoalveolar lavage samples of six dogs with respiratory disease, from two separate kennels in the United Kingdom. The genetic similarity of the isolates was investigated using pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD). Most of the isolates from four dogs housed at a re-homing kennel were genetically similar and some isolates from different dogs were indistinguishable by both PFGE and RAPD. These isolates were cultured from dogs with non-overlapping stays in the kennel, which may indicate maintenance of some strains within kennels. A small number of isolates showed much greater genetic heterogeneity and were genetically distinct from the main group of M. cynos strains. There was also a high degree of similarity of the M. cynos type strain (isolated from a dog with respiratory disease in Denmark in 1971) to at least one of the United Kingdom isolates using PFGE analysis, which may suggest possible conservation of pathogenic strains of M. cynos.
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spelling pubmed-71269312020-04-08 Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease Mannering, Sally A. McAuliffe, Laura Lawes, Joanna R. Erles, Kerstin Brownlie, Joe Vet Microbiol Article The association of Mycoplasma cynos with canine infectious respiratory disease is increasingly being recognised. This study describes the strain typing of 14 M. cynos isolates cultured from trachea and bronchoalveolar lavage samples of six dogs with respiratory disease, from two separate kennels in the United Kingdom. The genetic similarity of the isolates was investigated using pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD). Most of the isolates from four dogs housed at a re-homing kennel were genetically similar and some isolates from different dogs were indistinguishable by both PFGE and RAPD. These isolates were cultured from dogs with non-overlapping stays in the kennel, which may indicate maintenance of some strains within kennels. A small number of isolates showed much greater genetic heterogeneity and were genetically distinct from the main group of M. cynos strains. There was also a high degree of similarity of the M. cynos type strain (isolated from a dog with respiratory disease in Denmark in 1971) to at least one of the United Kingdom isolates using PFGE analysis, which may suggest possible conservation of pathogenic strains of M. cynos. Published by Elsevier B.V. 2009-03-30 2008-09-21 /pmc/articles/PMC7126931/ /pubmed/18977617 http://dx.doi.org/10.1016/j.vetmic.2008.09.058 Text en Crown copyright © 2008 Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Mannering, Sally A.
McAuliffe, Laura
Lawes, Joanna R.
Erles, Kerstin
Brownlie, Joe
Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease
title Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease
title_full Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease
title_fullStr Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease
title_full_unstemmed Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease
title_short Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease
title_sort strain typing of mycoplasma cynos isolates from dogs with respiratory disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126931/
https://www.ncbi.nlm.nih.gov/pubmed/18977617
http://dx.doi.org/10.1016/j.vetmic.2008.09.058
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