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Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions
Porcine epidemic diarrhea virus (PEDV) causes a severe clinical enteric disease in suckling neonates with up to 100% mortality, resulting in devastating economic losses to the pork industry in recent years. Maternal immunity via colostrum and milk is a vital source to neonates of passive protection...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126973/ https://www.ncbi.nlm.nih.gov/pubmed/27287711 http://dx.doi.org/10.1016/j.virusres.2016.06.002 |
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author | Song, Qinye Stone, Suzanne Drebes, Donna Greiner, Laura L. Dvorak, Cheryl M.T. Murtaugh, Michael P. |
author_facet | Song, Qinye Stone, Suzanne Drebes, Donna Greiner, Laura L. Dvorak, Cheryl M.T. Murtaugh, Michael P. |
author_sort | Song, Qinye |
collection | PubMed |
description | Porcine epidemic diarrhea virus (PEDV) causes a severe clinical enteric disease in suckling neonates with up to 100% mortality, resulting in devastating economic losses to the pork industry in recent years. Maternal immunity via colostrum and milk is a vital source to neonates of passive protection against diarrhea, dehydration and death caused by PEDV. Comprehensive information on neutralizing activity (NA) against PEDV in mammary secretions is critically important for assessing the protective capacity of sows. Therefore, the objectives of this study were to characterize anti-PEDV neutralizing activity in mammary secretions. Anti-PEDV NA was present in colostrum, milk and serum from PEDV-infected sows as determined both by immunofluorescence and ELISA-based neutralizing assays, with neutralization levels higher in colostrum and milk than in serum. The highest NA was observed in colostrum on day 1, and decreased rapidly in milk at day 3, then gradually declined from day 3 to day 19 post-farrowing. Notably, the NA in mammary secretions showed various patterns of decline over time of lactation that may contribute to variation in sow protective capacities. The kinetics of NA decline were associated with total IgA and IgG antibody levels. Neutralizing activity significantly correlated with specific IgA primarily to spike domain 1 (S1) and domain 2 (S2) proteins of PEDV rather than to specific IgG in colostrum. Subsequently, the NA in milk was mainly related to specific IgA to S1 and S2 during lactation. |
format | Online Article Text |
id | pubmed-7126973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71269732020-04-08 Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions Song, Qinye Stone, Suzanne Drebes, Donna Greiner, Laura L. Dvorak, Cheryl M.T. Murtaugh, Michael P. Virus Res Article Porcine epidemic diarrhea virus (PEDV) causes a severe clinical enteric disease in suckling neonates with up to 100% mortality, resulting in devastating economic losses to the pork industry in recent years. Maternal immunity via colostrum and milk is a vital source to neonates of passive protection against diarrhea, dehydration and death caused by PEDV. Comprehensive information on neutralizing activity (NA) against PEDV in mammary secretions is critically important for assessing the protective capacity of sows. Therefore, the objectives of this study were to characterize anti-PEDV neutralizing activity in mammary secretions. Anti-PEDV NA was present in colostrum, milk and serum from PEDV-infected sows as determined both by immunofluorescence and ELISA-based neutralizing assays, with neutralization levels higher in colostrum and milk than in serum. The highest NA was observed in colostrum on day 1, and decreased rapidly in milk at day 3, then gradually declined from day 3 to day 19 post-farrowing. Notably, the NA in mammary secretions showed various patterns of decline over time of lactation that may contribute to variation in sow protective capacities. The kinetics of NA decline were associated with total IgA and IgG antibody levels. Neutralizing activity significantly correlated with specific IgA primarily to spike domain 1 (S1) and domain 2 (S2) proteins of PEDV rather than to specific IgG in colostrum. Subsequently, the NA in milk was mainly related to specific IgA to S1 and S2 during lactation. Elsevier B.V. 2016-12-02 2016-06-07 /pmc/articles/PMC7126973/ /pubmed/27287711 http://dx.doi.org/10.1016/j.virusres.2016.06.002 Text en © 2016 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Song, Qinye Stone, Suzanne Drebes, Donna Greiner, Laura L. Dvorak, Cheryl M.T. Murtaugh, Michael P. Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions |
title | Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions |
title_full | Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions |
title_fullStr | Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions |
title_full_unstemmed | Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions |
title_short | Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions |
title_sort | characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126973/ https://www.ncbi.nlm.nih.gov/pubmed/27287711 http://dx.doi.org/10.1016/j.virusres.2016.06.002 |
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