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Sequencing of prototype viruses in the Venezuelan equine encephalitis antigenic complex

The 5′ nontranslated region (5′NTR) and nonstructural region nucleotide sequences of nine enzootic Venezuelan equine encephalitis (VEE) virus strains were determined, thus completing the genomic RNA sequences of all prototype strains. The full-length genomes, representing VEE virus antigenic subtype...

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Autores principales: Meissner, John D., Huang, Claire Y.-H., Pfeffer, Martin, Kinney, Richard M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B.V. 1999
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126981/
https://www.ncbi.nlm.nih.gov/pubmed/10500282
http://dx.doi.org/10.1016/S0168-1702(99)00078-7
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author Meissner, John D.
Huang, Claire Y.-H.
Pfeffer, Martin
Kinney, Richard M.
author_facet Meissner, John D.
Huang, Claire Y.-H.
Pfeffer, Martin
Kinney, Richard M.
author_sort Meissner, John D.
collection PubMed
description The 5′ nontranslated region (5′NTR) and nonstructural region nucleotide sequences of nine enzootic Venezuelan equine encephalitis (VEE) virus strains were determined, thus completing the genomic RNA sequences of all prototype strains. The full-length genomes, representing VEE virus antigenic subtypes I–VI, range in size from 11.3 to 11.5 kilobases, with 48–53% overall G+C contents. Size disparities result from subtype-related differences in the number and length of direct repeats in the C-terminal nonstructural protein 3 (nsP3) domain coding sequence and the 3′NTR, while G+C content disparities are attributable to strain-specific variations in base composition at the wobble position of the polyprotein codons. Highly-conserved protein components and one nonconserved protein domain constitute the VEE virus replicase polyproteins. Approximately 80% of deduced nsP1 and nsP4 amino acid residues are invariant, compared to less than 20% of C-terminal nsP3 domain residues. In two enzootic strains, C-terminal nsP3 domain sequences degenerate into little more than repetitive serine-rich blocks. Nonstructural region sequence information drawn from a cross-section of VEE virus subtypes clarifies features of alphavirus conserved sequence elements and proteinase recognition signals. As well, whole-genome comparative analysis supports the reclassification of VEE subtype-variety IF and subtype II viruses.
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spelling pubmed-71269812020-04-08 Sequencing of prototype viruses in the Venezuelan equine encephalitis antigenic complex Meissner, John D. Huang, Claire Y.-H. Pfeffer, Martin Kinney, Richard M. Virus Res Article The 5′ nontranslated region (5′NTR) and nonstructural region nucleotide sequences of nine enzootic Venezuelan equine encephalitis (VEE) virus strains were determined, thus completing the genomic RNA sequences of all prototype strains. The full-length genomes, representing VEE virus antigenic subtypes I–VI, range in size from 11.3 to 11.5 kilobases, with 48–53% overall G+C contents. Size disparities result from subtype-related differences in the number and length of direct repeats in the C-terminal nonstructural protein 3 (nsP3) domain coding sequence and the 3′NTR, while G+C content disparities are attributable to strain-specific variations in base composition at the wobble position of the polyprotein codons. Highly-conserved protein components and one nonconserved protein domain constitute the VEE virus replicase polyproteins. Approximately 80% of deduced nsP1 and nsP4 amino acid residues are invariant, compared to less than 20% of C-terminal nsP3 domain residues. In two enzootic strains, C-terminal nsP3 domain sequences degenerate into little more than repetitive serine-rich blocks. Nonstructural region sequence information drawn from a cross-section of VEE virus subtypes clarifies features of alphavirus conserved sequence elements and proteinase recognition signals. As well, whole-genome comparative analysis supports the reclassification of VEE subtype-variety IF and subtype II viruses. Elsevier Science B.V. 1999-10 1999-09-23 /pmc/articles/PMC7126981/ /pubmed/10500282 http://dx.doi.org/10.1016/S0168-1702(99)00078-7 Text en Copyright © 1999 Elsevier Science B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Meissner, John D.
Huang, Claire Y.-H.
Pfeffer, Martin
Kinney, Richard M.
Sequencing of prototype viruses in the Venezuelan equine encephalitis antigenic complex
title Sequencing of prototype viruses in the Venezuelan equine encephalitis antigenic complex
title_full Sequencing of prototype viruses in the Venezuelan equine encephalitis antigenic complex
title_fullStr Sequencing of prototype viruses in the Venezuelan equine encephalitis antigenic complex
title_full_unstemmed Sequencing of prototype viruses in the Venezuelan equine encephalitis antigenic complex
title_short Sequencing of prototype viruses in the Venezuelan equine encephalitis antigenic complex
title_sort sequencing of prototype viruses in the venezuelan equine encephalitis antigenic complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126981/
https://www.ncbi.nlm.nih.gov/pubmed/10500282
http://dx.doi.org/10.1016/S0168-1702(99)00078-7
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