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Canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosis

Canine distemper virus (CDV), a negative stranded RNA morbillivirus, causes a multisystemic disease in dogs, which is associated with a severe immune suppression. The aim of the study was to examine the influence of early CDV infection on leukocyte depletion, lymphopenia and virus-induced cell death...

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Autores principales: Schobesberger, Martina, Summerfield, Artur, Doherr, Marcus G., Zurbriggen, Andreas, Griot, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127010/
https://www.ncbi.nlm.nih.gov/pubmed/15661329
http://dx.doi.org/10.1016/j.vetimm.2004.09.032
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author Schobesberger, Martina
Summerfield, Artur
Doherr, Marcus G.
Zurbriggen, Andreas
Griot, Christian
author_facet Schobesberger, Martina
Summerfield, Artur
Doherr, Marcus G.
Zurbriggen, Andreas
Griot, Christian
author_sort Schobesberger, Martina
collection PubMed
description Canine distemper virus (CDV), a negative stranded RNA morbillivirus, causes a multisystemic disease in dogs, which is associated with a severe immune suppression. The aim of the study was to examine the influence of early CDV infection on leukocyte depletion, lymphopenia and virus-induced cell death in dogs infected with a virulent CDV strain. From 10 infected dogs, peripheral blood leukocytes were harvested periodically, phenotyped and analyzed for CDV antigen content and apoptosis using Annexin V-FITC and propidium iodide labeling. CDV infection induced a severe CD3(+) T cell and CD21(+) B cell depletion in all animals at 3 days post-infection (d.p.i.). For dogs with severe distemper, developing virus persistence in the lymphoid tissue and central nervous system, this lymphopenia lasted until the end of the experiment. Increased levels of lymphocyte apoptosis were found at 3 d.p.i., and monocyte apoptosis at 6 d.p.i. This was more prominent in the group of animals with severe distemper. At 3 d.p.i. no leukocyte infection was detectable indicating that the early lymphocyte depletion and apoptosis was not a direct consequence of virus infection. Taken together, our results demonstrate that CDV-induced lymphopenia is an early event and that the degree of lymphocyte depletion correlates with the severity of disease and virus persistence in the lymphoid tissue and central nervous system.
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spelling pubmed-71270102020-04-08 Canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosis Schobesberger, Martina Summerfield, Artur Doherr, Marcus G. Zurbriggen, Andreas Griot, Christian Vet Immunol Immunopathol Article Canine distemper virus (CDV), a negative stranded RNA morbillivirus, causes a multisystemic disease in dogs, which is associated with a severe immune suppression. The aim of the study was to examine the influence of early CDV infection on leukocyte depletion, lymphopenia and virus-induced cell death in dogs infected with a virulent CDV strain. From 10 infected dogs, peripheral blood leukocytes were harvested periodically, phenotyped and analyzed for CDV antigen content and apoptosis using Annexin V-FITC and propidium iodide labeling. CDV infection induced a severe CD3(+) T cell and CD21(+) B cell depletion in all animals at 3 days post-infection (d.p.i.). For dogs with severe distemper, developing virus persistence in the lymphoid tissue and central nervous system, this lymphopenia lasted until the end of the experiment. Increased levels of lymphocyte apoptosis were found at 3 d.p.i., and monocyte apoptosis at 6 d.p.i. This was more prominent in the group of animals with severe distemper. At 3 d.p.i. no leukocyte infection was detectable indicating that the early lymphocyte depletion and apoptosis was not a direct consequence of virus infection. Taken together, our results demonstrate that CDV-induced lymphopenia is an early event and that the degree of lymphocyte depletion correlates with the severity of disease and virus persistence in the lymphoid tissue and central nervous system. Elsevier B.V. 2005-03-10 2004-12-18 /pmc/articles/PMC7127010/ /pubmed/15661329 http://dx.doi.org/10.1016/j.vetimm.2004.09.032 Text en Copyright © 2004 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Schobesberger, Martina
Summerfield, Artur
Doherr, Marcus G.
Zurbriggen, Andreas
Griot, Christian
Canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosis
title Canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosis
title_full Canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosis
title_fullStr Canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosis
title_full_unstemmed Canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosis
title_short Canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosis
title_sort canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127010/
https://www.ncbi.nlm.nih.gov/pubmed/15661329
http://dx.doi.org/10.1016/j.vetimm.2004.09.032
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