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High-level prokaryotic expression of envelope exterior of membrane protein of porcine epidemic diarrhea virus

The truncated fragment M′ gene, encoding the exterior of the viral envelope protein of PEDV, was subcloned into prokaryotic expression vector pGEX-6p-1. The recombinant plasmid pGEX-6p-M′ was constructed and transformed into E. coli BL21(DE3)pLysS for expression. SDS-PAGE analysis showed recombinant...

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Detalles Bibliográficos
Autores principales:	Shenyang, Gao, Enhui, Zha, Baoxian, Li, Xinyuan, Qiao, Lijie, Tang, Junwei, Ge, Yijing, Li
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2007
Materias:	Short Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127142/ https://www.ncbi.nlm.nih.gov/pubmed/17475420 http://dx.doi.org/10.1016/j.vetmic.2007.03.027

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