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Infection of dogs by experimental inoculation with human isolates of Giardia duodenalis: Clinical and laboratory manifestations
The susceptibility of dogs to experimental inoculation with trophozoites and cysts of human isolates of Giardia duodenalis and the clinical and laboratory profiles of infection of these animals were studied. Two groups (A and B), each comprising three dogs, were inoculated with G. duodenalis trophoz...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127194/ https://www.ncbi.nlm.nih.gov/pubmed/17178439 http://dx.doi.org/10.1016/j.vetpar.2006.11.016 |
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author | Rosa, L.A.G. Gomes, M.A. Mundim, A.V. Mundim, M.J.S. Pozzer, E.L. Faria, E.S.M. Viana, J.C. Cury, M.C. |
author_facet | Rosa, L.A.G. Gomes, M.A. Mundim, A.V. Mundim, M.J.S. Pozzer, E.L. Faria, E.S.M. Viana, J.C. Cury, M.C. |
author_sort | Rosa, L.A.G. |
collection | PubMed |
description | The susceptibility of dogs to experimental inoculation with trophozoites and cysts of human isolates of Giardia duodenalis and the clinical and laboratory profiles of infection of these animals were studied. Two groups (A and B), each comprising three dogs, were inoculated with G. duodenalis trophozoites and cysts, respectively. A third group of two dogs was not inoculated and remained as control. After inoculation feces were collected daily to determine the pre-patent period, by flotation in 33% zinc sulfate solution. Blood samples (5 mL) were collected from animals at 15-day intervals during the 165 days of the experimental period and were used to carry out the hemogram and biochemical evaluation of the levels of total protein, albumin, alanine aminotransferase, gamma glutamyltransferase, urea and creatinine. A prepatent period was observed at 5–6 days post-inoculation (p.i.) in the inoculated dogs, with cysts eliminated for approximately 3 months. No alterations were seen in the clinical parameters evaluated. Anemia was observed at 15 p.i. in the inoculated dogs. The mean eosinophil count of inoculated groups was higher than that of the control (p ≤ 0.05) but none of the biochemical parameters analyzed presented significant differences. The results of this study show that G. duodenalis from human isolates is able to infect dogs with minimal systemic manifestations without producing clinical signs of giardiasis. |
format | Online Article Text |
id | pubmed-7127194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71271942020-04-08 Infection of dogs by experimental inoculation with human isolates of Giardia duodenalis: Clinical and laboratory manifestations Rosa, L.A.G. Gomes, M.A. Mundim, A.V. Mundim, M.J.S. Pozzer, E.L. Faria, E.S.M. Viana, J.C. Cury, M.C. Vet Parasitol Article The susceptibility of dogs to experimental inoculation with trophozoites and cysts of human isolates of Giardia duodenalis and the clinical and laboratory profiles of infection of these animals were studied. Two groups (A and B), each comprising three dogs, were inoculated with G. duodenalis trophozoites and cysts, respectively. A third group of two dogs was not inoculated and remained as control. After inoculation feces were collected daily to determine the pre-patent period, by flotation in 33% zinc sulfate solution. Blood samples (5 mL) were collected from animals at 15-day intervals during the 165 days of the experimental period and were used to carry out the hemogram and biochemical evaluation of the levels of total protein, albumin, alanine aminotransferase, gamma glutamyltransferase, urea and creatinine. A prepatent period was observed at 5–6 days post-inoculation (p.i.) in the inoculated dogs, with cysts eliminated for approximately 3 months. No alterations were seen in the clinical parameters evaluated. Anemia was observed at 15 p.i. in the inoculated dogs. The mean eosinophil count of inoculated groups was higher than that of the control (p ≤ 0.05) but none of the biochemical parameters analyzed presented significant differences. The results of this study show that G. duodenalis from human isolates is able to infect dogs with minimal systemic manifestations without producing clinical signs of giardiasis. Elsevier B.V. 2007-04-10 2006-12-18 /pmc/articles/PMC7127194/ /pubmed/17178439 http://dx.doi.org/10.1016/j.vetpar.2006.11.016 Text en Copyright © 2006 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Rosa, L.A.G. Gomes, M.A. Mundim, A.V. Mundim, M.J.S. Pozzer, E.L. Faria, E.S.M. Viana, J.C. Cury, M.C. Infection of dogs by experimental inoculation with human isolates of Giardia duodenalis: Clinical and laboratory manifestations |
title | Infection of dogs by experimental inoculation with human isolates of Giardia duodenalis: Clinical and laboratory manifestations |
title_full | Infection of dogs by experimental inoculation with human isolates of Giardia duodenalis: Clinical and laboratory manifestations |
title_fullStr | Infection of dogs by experimental inoculation with human isolates of Giardia duodenalis: Clinical and laboratory manifestations |
title_full_unstemmed | Infection of dogs by experimental inoculation with human isolates of Giardia duodenalis: Clinical and laboratory manifestations |
title_short | Infection of dogs by experimental inoculation with human isolates of Giardia duodenalis: Clinical and laboratory manifestations |
title_sort | infection of dogs by experimental inoculation with human isolates of giardia duodenalis: clinical and laboratory manifestations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127194/ https://www.ncbi.nlm.nih.gov/pubmed/17178439 http://dx.doi.org/10.1016/j.vetpar.2006.11.016 |
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