Cargando…
Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica
Bovine respiratory disease (BRD) often occurs when active respiratory virus infections (BHV-1, etc.) impair resistance to Mannheimia haemolytica infection in the lower respiratory tract. The interactions that occur when the respiratory epithelium encounters these viral and bacterial pathogens are po...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127263/ https://www.ncbi.nlm.nih.gov/pubmed/23890750 http://dx.doi.org/10.1016/j.vetimm.2013.06.012 |
_version_ | 1783516322237251584 |
---|---|
author | N’jai, Alhaji U. Rivera, Jose Atapattu, Dhammika N. Owusu-Ofori, Kwadwo Czuprynski, Charles J. |
author_facet | N’jai, Alhaji U. Rivera, Jose Atapattu, Dhammika N. Owusu-Ofori, Kwadwo Czuprynski, Charles J. |
author_sort | N’jai, Alhaji U. |
collection | PubMed |
description | Bovine respiratory disease (BRD) often occurs when active respiratory virus infections (BHV-1, etc.) impair resistance to Mannheimia haemolytica infection in the lower respiratory tract. The interactions that occur when the respiratory epithelium encounters these viral and bacterial pathogens are poorly understood. We used Agilent bovine gene microarray chips containing 44,000 transcripts to elucidate bovine bronchial epithelial cell (BBEC) responses following in vitro exposure to BHV-1 alone, M. haemolytica alone, or both BHV-1 and M. haemolytica. Microarray analysis revealed differential regulation (>2-fold) of 978 transcripts by BHV-1 alone, 2040 transcripts by M. haemolytica alone, and 2189 genes by BHV-1 and M. haemolytica in combination. M. haemolytica treatment produced significantly greater inductions (>10-fold) of several inflammation associated genes, such as CXCL2, IL-6, IL-1α, e-selectin, and IL-8, than to BHV-1 alone. Functional analysis of the microarray data revealed a significant upregulation of genes involved in important biological processes such as inflammation (TNF-α, IL-8, Tlr-2, IL-1, CXCL2, CSF2), vascular functions (VEGF, EDN2) and leukocyte migration (ICAM1, IL-16) during a co-infection with BHV-1 and M. haemolytica compared to either pathogen alone. This study provides evidence to support that lung epithelial cells are a source of mediators that may promote inflammatory changes observed during bovine respiratory disease. |
format | Online Article Text |
id | pubmed-7127263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71272632020-04-08 Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica N’jai, Alhaji U. Rivera, Jose Atapattu, Dhammika N. Owusu-Ofori, Kwadwo Czuprynski, Charles J. Vet Immunol Immunopathol Article Bovine respiratory disease (BRD) often occurs when active respiratory virus infections (BHV-1, etc.) impair resistance to Mannheimia haemolytica infection in the lower respiratory tract. The interactions that occur when the respiratory epithelium encounters these viral and bacterial pathogens are poorly understood. We used Agilent bovine gene microarray chips containing 44,000 transcripts to elucidate bovine bronchial epithelial cell (BBEC) responses following in vitro exposure to BHV-1 alone, M. haemolytica alone, or both BHV-1 and M. haemolytica. Microarray analysis revealed differential regulation (>2-fold) of 978 transcripts by BHV-1 alone, 2040 transcripts by M. haemolytica alone, and 2189 genes by BHV-1 and M. haemolytica in combination. M. haemolytica treatment produced significantly greater inductions (>10-fold) of several inflammation associated genes, such as CXCL2, IL-6, IL-1α, e-selectin, and IL-8, than to BHV-1 alone. Functional analysis of the microarray data revealed a significant upregulation of genes involved in important biological processes such as inflammation (TNF-α, IL-8, Tlr-2, IL-1, CXCL2, CSF2), vascular functions (VEGF, EDN2) and leukocyte migration (ICAM1, IL-16) during a co-infection with BHV-1 and M. haemolytica compared to either pathogen alone. This study provides evidence to support that lung epithelial cells are a source of mediators that may promote inflammatory changes observed during bovine respiratory disease. Elsevier B.V. 2013-09-15 2013-07-01 /pmc/articles/PMC7127263/ /pubmed/23890750 http://dx.doi.org/10.1016/j.vetimm.2013.06.012 Text en Copyright © 2013 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article N’jai, Alhaji U. Rivera, Jose Atapattu, Dhammika N. Owusu-Ofori, Kwadwo Czuprynski, Charles J. Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica |
title | Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica |
title_full | Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica |
title_fullStr | Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica |
title_full_unstemmed | Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica |
title_short | Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica |
title_sort | gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and mannheimia haemolytica |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127263/ https://www.ncbi.nlm.nih.gov/pubmed/23890750 http://dx.doi.org/10.1016/j.vetimm.2013.06.012 |
work_keys_str_mv | AT njaialhajiu geneexpressionprofilingofbovinebronchialepithelialcellsexposedinvitrotobovineherpesvirus1andmannheimiahaemolytica AT riverajose geneexpressionprofilingofbovinebronchialepithelialcellsexposedinvitrotobovineherpesvirus1andmannheimiahaemolytica AT atapattudhammikan geneexpressionprofilingofbovinebronchialepithelialcellsexposedinvitrotobovineherpesvirus1andmannheimiahaemolytica AT owusuoforikwadwo geneexpressionprofilingofbovinebronchialepithelialcellsexposedinvitrotobovineherpesvirus1andmannheimiahaemolytica AT czuprynskicharlesj geneexpressionprofilingofbovinebronchialepithelialcellsexposedinvitrotobovineherpesvirus1andmannheimiahaemolytica |