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Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles

The development and characterization of a quartz crystal microbalance (QCM) sensor for the direct detection of aerosolized influenza A virions is reported. Self-assembled monolayers (SAMs) of mercaptoundecanoic acid (MUA) are formed on QCM gold electrodes to provide a surface amenable for the immobi...

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Autores principales: Owen, Thomas W., Al-Kaysi, Rabih O., Bardeen, Christopher J., Cheng, Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127275/
https://www.ncbi.nlm.nih.gov/pubmed/32288239
http://dx.doi.org/10.1016/j.snb.2007.04.028
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author Owen, Thomas W.
Al-Kaysi, Rabih O.
Bardeen, Christopher J.
Cheng, Quan
author_facet Owen, Thomas W.
Al-Kaysi, Rabih O.
Bardeen, Christopher J.
Cheng, Quan
author_sort Owen, Thomas W.
collection PubMed
description The development and characterization of a quartz crystal microbalance (QCM) sensor for the direct detection of aerosolized influenza A virions is reported. Self-assembled monolayers (SAMs) of mercaptoundecanoic acid (MUA) are formed on QCM gold electrodes to provide a surface amenable for the immobilization of anti-influenza A antibodies using NHS/EDC coupling chemistry. The surface-bound antibody provides a selective and specific sensing interface for the capture of influenza virions. A nebulizer is used to create aerosolized samples and is directly connected to a chamber housing the antibody-modified crystal (“immunochip”). Upon exposure to the aerosolized virus, the interaction between the antibody and virus leads to a dampening of the oscillation frequency of the quartz crystal. The magnitude of frequency change is directly related to virus concentration. Control experiments using aerosols from chicken egg allantoic fluid and an anti-murine antibody based immunosensor confirm that the observed signal originates from specific viral binding on the chip surface. Step-by-step surface modification of MUA assembly, antibody attachment, and antibody–virus interaction are characterized by atomic force microscopy (AFM) imaging analysis. Using the S/N = 3 principle, the limit of detection is estimated to be 4 virus particles/mL. The high sensitivity and real-time sensing scheme presented here can play an important role in the public health arena by offering a new analytical tool for identifying bio-contaminated areas and assisting in timely patient diagnosis.
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spelling pubmed-71272752020-04-08 Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles Owen, Thomas W. Al-Kaysi, Rabih O. Bardeen, Christopher J. Cheng, Quan Sens Actuators B Chem Article The development and characterization of a quartz crystal microbalance (QCM) sensor for the direct detection of aerosolized influenza A virions is reported. Self-assembled monolayers (SAMs) of mercaptoundecanoic acid (MUA) are formed on QCM gold electrodes to provide a surface amenable for the immobilization of anti-influenza A antibodies using NHS/EDC coupling chemistry. The surface-bound antibody provides a selective and specific sensing interface for the capture of influenza virions. A nebulizer is used to create aerosolized samples and is directly connected to a chamber housing the antibody-modified crystal (“immunochip”). Upon exposure to the aerosolized virus, the interaction between the antibody and virus leads to a dampening of the oscillation frequency of the quartz crystal. The magnitude of frequency change is directly related to virus concentration. Control experiments using aerosols from chicken egg allantoic fluid and an anti-murine antibody based immunosensor confirm that the observed signal originates from specific viral binding on the chip surface. Step-by-step surface modification of MUA assembly, antibody attachment, and antibody–virus interaction are characterized by atomic force microscopy (AFM) imaging analysis. Using the S/N = 3 principle, the limit of detection is estimated to be 4 virus particles/mL. The high sensitivity and real-time sensing scheme presented here can play an important role in the public health arena by offering a new analytical tool for identifying bio-contaminated areas and assisting in timely patient diagnosis. Elsevier B.V. 2007-10-01 2007-04-24 /pmc/articles/PMC7127275/ /pubmed/32288239 http://dx.doi.org/10.1016/j.snb.2007.04.028 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Owen, Thomas W.
Al-Kaysi, Rabih O.
Bardeen, Christopher J.
Cheng, Quan
Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles
title Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles
title_full Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles
title_fullStr Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles
title_full_unstemmed Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles
title_short Microgravimetric immunosensor for direct detection of aerosolized influenza A virus particles
title_sort microgravimetric immunosensor for direct detection of aerosolized influenza a virus particles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127275/
https://www.ncbi.nlm.nih.gov/pubmed/32288239
http://dx.doi.org/10.1016/j.snb.2007.04.028
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