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Three-color flow cytometry detection of virus-specific CD4+ and CD8+ T cells in the cat

We describe a three-color flow cytometry assay for the detection of virus-specific CD4+ and CD8+ T cells in the cat. The assay is based upon detection of intracellular TNFα using the cross-reactive mAb 6401.1111, raised against the human cytokine. Allophycocyanin-conjugated mAb 6401.1111 specificall...

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Detalles Bibliográficos
Autores principales:	de Groot-Mijnes, Jolanda D.F., van der Most, Robbert G., van Dun, Jessica M., te Lintelo, Eddie G., Schuurman, Nancy M.P., Egberink, Herman F., de Groot, Raoul J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier B.V. 2004
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127382/ https://www.ncbi.nlm.nih.gov/pubmed/14871533 http://dx.doi.org/10.1016/j.jim.2003.10.019

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