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Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species
The demand for diagnostic tools that allow simultaneous screening of samples for multiple pathogens is increasing because they overcome the limitations of other methods, which can only screen for a single or a few pathogens at a time. Microarrays offer the advantages of being capable to test a large...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127396/ https://www.ncbi.nlm.nih.gov/pubmed/26188129 http://dx.doi.org/10.1016/j.mcp.2015.07.005 |
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author | Giles, Timothy Yon, Lisa Hannant, Duncan Barrow, Paul Abu-Median, Abu-Bakr |
author_facet | Giles, Timothy Yon, Lisa Hannant, Duncan Barrow, Paul Abu-Median, Abu-Bakr |
author_sort | Giles, Timothy |
collection | PubMed |
description | The demand for diagnostic tools that allow simultaneous screening of samples for multiple pathogens is increasing because they overcome the limitations of other methods, which can only screen for a single or a few pathogens at a time. Microarrays offer the advantages of being capable to test a large number of samples simultaneously, screening for multiple pathogen types per sample and having comparable sensitivity to existing methods such as PCR. Array design is often considered the most important process in any microarray experiment and can be the deciding factor in the success of a study. There are currently no microarrays for simultaneous detection of rodent-borne pathogens. The aim of this report is to explicate the design, development and evaluation of a microarray platform for use as a screening tool that combines ease of use and rapid identification of a number of rodent-borne pathogens of zoonotic importance. Nucleic acid was amplified by multiplex biotinylation PCR prior to hybridisation onto microarrays. The array sensitivity was comparable to standard PCR, though less sensitive than real-time PCR. The array presented here is a prototype microarray identification system for zoonotic pathogens that can infect rodent species. |
format | Online Article Text |
id | pubmed-7127396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71273962020-04-08 Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species Giles, Timothy Yon, Lisa Hannant, Duncan Barrow, Paul Abu-Median, Abu-Bakr Mol Cell Probes Original Research Article The demand for diagnostic tools that allow simultaneous screening of samples for multiple pathogens is increasing because they overcome the limitations of other methods, which can only screen for a single or a few pathogens at a time. Microarrays offer the advantages of being capable to test a large number of samples simultaneously, screening for multiple pathogen types per sample and having comparable sensitivity to existing methods such as PCR. Array design is often considered the most important process in any microarray experiment and can be the deciding factor in the success of a study. There are currently no microarrays for simultaneous detection of rodent-borne pathogens. The aim of this report is to explicate the design, development and evaluation of a microarray platform for use as a screening tool that combines ease of use and rapid identification of a number of rodent-borne pathogens of zoonotic importance. Nucleic acid was amplified by multiplex biotinylation PCR prior to hybridisation onto microarrays. The array sensitivity was comparable to standard PCR, though less sensitive than real-time PCR. The array presented here is a prototype microarray identification system for zoonotic pathogens that can infect rodent species. Elsevier Ltd. 2015-12 2015-07-15 /pmc/articles/PMC7127396/ /pubmed/26188129 http://dx.doi.org/10.1016/j.mcp.2015.07.005 Text en Copyright © 2015 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Original Research Article Giles, Timothy Yon, Lisa Hannant, Duncan Barrow, Paul Abu-Median, Abu-Bakr Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species |
title | Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species |
title_full | Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species |
title_fullStr | Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species |
title_full_unstemmed | Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species |
title_short | Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species |
title_sort | development of a dna-based microarray for the detection of zoonotic pathogens in rodent species |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127396/ https://www.ncbi.nlm.nih.gov/pubmed/26188129 http://dx.doi.org/10.1016/j.mcp.2015.07.005 |
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