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Mitochondria-Modulating Porous Se@SiO(2) Nanoparticles Provide Resistance to Oxidative Injury in Airway Epithelial Cells: Implications for Acute Lung Injury
BACKGROUND: Mitochondrial dysfunction played a vital role in the pathogenesis of various diseases, including acute lung injury (ALI). However, few strategies targeting mitochondria were developed in treating ALI. Recently, we fabricated a porous Se@SiO(2) nanoparticles (NPs) with antioxidant propert...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127826/ https://www.ncbi.nlm.nih.gov/pubmed/32280221 http://dx.doi.org/10.2147/IJN.S240301 |
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author | Wang, Muyun Wang, Kun Deng, Guoying Liu, Xijian Wu, Xiaodong Hu, Haiyang Zhang, Yanbei Gao, Wei Li, Qiang |
author_facet | Wang, Muyun Wang, Kun Deng, Guoying Liu, Xijian Wu, Xiaodong Hu, Haiyang Zhang, Yanbei Gao, Wei Li, Qiang |
author_sort | Wang, Muyun |
collection | PubMed |
description | BACKGROUND: Mitochondrial dysfunction played a vital role in the pathogenesis of various diseases, including acute lung injury (ALI). However, few strategies targeting mitochondria were developed in treating ALI. Recently, we fabricated a porous Se@SiO(2) nanoparticles (NPs) with antioxidant properties. METHODS: The protective effect of Se@SiO(2) NPs was assessed using confocal imaging, immunoblotting, RNA-seq, mitochondrial respiratory chain (MRC) activity assay, and transmission electron microscopy (TEM) in airway epithelial cell line (Beas-2B). The in vivo efficacy of Se@SiO(2) NPs was evaluated in a lipopolysaccharide (LPS)-induced ALI mouse model. RESULTS: This study demonstrated that Se@SiO(2) NPs significantly increased the resistance of airway epithelial cells under oxidative injury and shifted lipopolysaccharide-induced gene expression profile closer to the untreated controls. The cytoprotection of Se@SiO(2) was found to be achieved by maintaining mitochondrial function, activity, and dynamics. In an animal model of ALI, pretreated with the NPs improved mitochondrial dysfunction, thus reducing inflammatory responses and diffuse damage in lung tissues. Additionally, RNA-seq analysis provided evidence for the broad modulatory activity of our Se@SiO(2) NPs in various metabolic disorders and inflammatory diseases. CONCLUSION: This study brought new insights into mitochondria-targeting bioactive NPs, with application potential in curing ALI or other human mitochondria-related disorders. |
format | Online Article Text |
id | pubmed-7127826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-71278262020-04-10 Mitochondria-Modulating Porous Se@SiO(2) Nanoparticles Provide Resistance to Oxidative Injury in Airway Epithelial Cells: Implications for Acute Lung Injury Wang, Muyun Wang, Kun Deng, Guoying Liu, Xijian Wu, Xiaodong Hu, Haiyang Zhang, Yanbei Gao, Wei Li, Qiang Int J Nanomedicine Original Research BACKGROUND: Mitochondrial dysfunction played a vital role in the pathogenesis of various diseases, including acute lung injury (ALI). However, few strategies targeting mitochondria were developed in treating ALI. Recently, we fabricated a porous Se@SiO(2) nanoparticles (NPs) with antioxidant properties. METHODS: The protective effect of Se@SiO(2) NPs was assessed using confocal imaging, immunoblotting, RNA-seq, mitochondrial respiratory chain (MRC) activity assay, and transmission electron microscopy (TEM) in airway epithelial cell line (Beas-2B). The in vivo efficacy of Se@SiO(2) NPs was evaluated in a lipopolysaccharide (LPS)-induced ALI mouse model. RESULTS: This study demonstrated that Se@SiO(2) NPs significantly increased the resistance of airway epithelial cells under oxidative injury and shifted lipopolysaccharide-induced gene expression profile closer to the untreated controls. The cytoprotection of Se@SiO(2) was found to be achieved by maintaining mitochondrial function, activity, and dynamics. In an animal model of ALI, pretreated with the NPs improved mitochondrial dysfunction, thus reducing inflammatory responses and diffuse damage in lung tissues. Additionally, RNA-seq analysis provided evidence for the broad modulatory activity of our Se@SiO(2) NPs in various metabolic disorders and inflammatory diseases. CONCLUSION: This study brought new insights into mitochondria-targeting bioactive NPs, with application potential in curing ALI or other human mitochondria-related disorders. Dove 2020-03-31 /pmc/articles/PMC7127826/ /pubmed/32280221 http://dx.doi.org/10.2147/IJN.S240301 Text en © 2020 Wang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Wang, Muyun Wang, Kun Deng, Guoying Liu, Xijian Wu, Xiaodong Hu, Haiyang Zhang, Yanbei Gao, Wei Li, Qiang Mitochondria-Modulating Porous Se@SiO(2) Nanoparticles Provide Resistance to Oxidative Injury in Airway Epithelial Cells: Implications for Acute Lung Injury |
title | Mitochondria-Modulating Porous Se@SiO(2) Nanoparticles Provide Resistance to Oxidative Injury in Airway Epithelial Cells: Implications for Acute Lung Injury |
title_full | Mitochondria-Modulating Porous Se@SiO(2) Nanoparticles Provide Resistance to Oxidative Injury in Airway Epithelial Cells: Implications for Acute Lung Injury |
title_fullStr | Mitochondria-Modulating Porous Se@SiO(2) Nanoparticles Provide Resistance to Oxidative Injury in Airway Epithelial Cells: Implications for Acute Lung Injury |
title_full_unstemmed | Mitochondria-Modulating Porous Se@SiO(2) Nanoparticles Provide Resistance to Oxidative Injury in Airway Epithelial Cells: Implications for Acute Lung Injury |
title_short | Mitochondria-Modulating Porous Se@SiO(2) Nanoparticles Provide Resistance to Oxidative Injury in Airway Epithelial Cells: Implications for Acute Lung Injury |
title_sort | mitochondria-modulating porous se@sio(2) nanoparticles provide resistance to oxidative injury in airway epithelial cells: implications for acute lung injury |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127826/ https://www.ncbi.nlm.nih.gov/pubmed/32280221 http://dx.doi.org/10.2147/IJN.S240301 |
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