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Genetic relatedness of serial rectal isolates of Acinetobacter baumannii in an adult intensive care unit of a tertiary hospital in Kuwait
Acinetobacter baumannii is an opportunistic pathogen of intensive care unit (ICU) patients. A. baumannii colonizes many parts of the body including the gastrointestinal tract. Endemic and epidemic strains are polyclonal. There is no clarity on the origin of polyclonality of A. baumannii. The objecti...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127897/ https://www.ncbi.nlm.nih.gov/pubmed/32240218 http://dx.doi.org/10.1371/journal.pone.0230976 |
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author | Al-Hashem, Ghayda Rotimi, Vincent O. Albert, M. John |
author_facet | Al-Hashem, Ghayda Rotimi, Vincent O. Albert, M. John |
author_sort | Al-Hashem, Ghayda |
collection | PubMed |
description | Acinetobacter baumannii is an opportunistic pathogen of intensive care unit (ICU) patients. A. baumannii colonizes many parts of the body including the gastrointestinal tract. Endemic and epidemic strains are polyclonal. There is no clarity on the origin of polyclonality of A. baumannii. The objective of the study was to define the genetic relatedness of serial isolates and the origin of polyclonality. Serial rectal isolates from ICU patients whose rectum was colonized on ≥5 sampling occasions were selected. From a total of 32 eligible colonized patients, isolates from a subgroup of 13 patients (a total of 108 isolates) showing different patterns of colonization as revealed by pulsed-field gel electrophoresis (PFGE) were studied. The isolates were analyzed by PFGE pulsotypes, sequence types (STs) by multi-locus sequence typing (MLST) and clonal complex (CC) by eBURST analysis. Serial isolates constituted a mixture of identical, related and unrelated pulsotypes. Analysis by STs and CCs were less discriminatory. The data suggest a combination of an initial colonizing isolate undergoing mutation as well as colonization by independent isolates. Further clarity on the origin of diversity should be better obtained by whole-genome sequencing. |
format | Online Article Text |
id | pubmed-7127897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-71278972020-04-09 Genetic relatedness of serial rectal isolates of Acinetobacter baumannii in an adult intensive care unit of a tertiary hospital in Kuwait Al-Hashem, Ghayda Rotimi, Vincent O. Albert, M. John PLoS One Research Article Acinetobacter baumannii is an opportunistic pathogen of intensive care unit (ICU) patients. A. baumannii colonizes many parts of the body including the gastrointestinal tract. Endemic and epidemic strains are polyclonal. There is no clarity on the origin of polyclonality of A. baumannii. The objective of the study was to define the genetic relatedness of serial isolates and the origin of polyclonality. Serial rectal isolates from ICU patients whose rectum was colonized on ≥5 sampling occasions were selected. From a total of 32 eligible colonized patients, isolates from a subgroup of 13 patients (a total of 108 isolates) showing different patterns of colonization as revealed by pulsed-field gel electrophoresis (PFGE) were studied. The isolates were analyzed by PFGE pulsotypes, sequence types (STs) by multi-locus sequence typing (MLST) and clonal complex (CC) by eBURST analysis. Serial isolates constituted a mixture of identical, related and unrelated pulsotypes. Analysis by STs and CCs were less discriminatory. The data suggest a combination of an initial colonizing isolate undergoing mutation as well as colonization by independent isolates. Further clarity on the origin of diversity should be better obtained by whole-genome sequencing. Public Library of Science 2020-04-02 /pmc/articles/PMC7127897/ /pubmed/32240218 http://dx.doi.org/10.1371/journal.pone.0230976 Text en © 2020 Al-Hashem et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Al-Hashem, Ghayda Rotimi, Vincent O. Albert, M. John Genetic relatedness of serial rectal isolates of Acinetobacter baumannii in an adult intensive care unit of a tertiary hospital in Kuwait |
title | Genetic relatedness of serial rectal isolates of
Acinetobacter baumannii in an adult intensive care unit of
a tertiary hospital in Kuwait |
title_full | Genetic relatedness of serial rectal isolates of
Acinetobacter baumannii in an adult intensive care unit of
a tertiary hospital in Kuwait |
title_fullStr | Genetic relatedness of serial rectal isolates of
Acinetobacter baumannii in an adult intensive care unit of
a tertiary hospital in Kuwait |
title_full_unstemmed | Genetic relatedness of serial rectal isolates of
Acinetobacter baumannii in an adult intensive care unit of
a tertiary hospital in Kuwait |
title_short | Genetic relatedness of serial rectal isolates of
Acinetobacter baumannii in an adult intensive care unit of
a tertiary hospital in Kuwait |
title_sort | genetic relatedness of serial rectal isolates of
acinetobacter baumannii in an adult intensive care unit of
a tertiary hospital in kuwait |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127897/ https://www.ncbi.nlm.nih.gov/pubmed/32240218 http://dx.doi.org/10.1371/journal.pone.0230976 |
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