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Indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients

BACKGROUND: Rapid and reliable diagnosis of respiratory viral infections (RVI) in lung transplant recipients is essential to direct therapy of acute graft dysfunction and identify epidemic trends. Traditional techniques of serology and viral culture are limited by the lack of antibody response and d...

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Autores principales: Hopkins, Peter M, Plit, Marshall L, Carter, Ian W, Chhajed, Prashant N, Malouf, Monique A, Glanville, Allan R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Society for Heart and Lung Transplantation. Published by Elsevier Inc. 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128093/
https://www.ncbi.nlm.nih.gov/pubmed/12581764
http://dx.doi.org/10.1016/S1053-2498(02)00467-9
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author Hopkins, Peter M
Plit, Marshall L
Carter, Ian W
Chhajed, Prashant N
Malouf, Monique A
Glanville, Allan R
author_facet Hopkins, Peter M
Plit, Marshall L
Carter, Ian W
Chhajed, Prashant N
Malouf, Monique A
Glanville, Allan R
author_sort Hopkins, Peter M
collection PubMed
description BACKGROUND: Rapid and reliable diagnosis of respiratory viral infections (RVI) in lung transplant recipients is essential to direct therapy of acute graft dysfunction and identify epidemic trends. Traditional techniques of serology and viral culture are limited by the lack of antibody response and delay in diagnosis. METHODS: We examined the clinical utility of indirect fluorescent antibody (IFA) testing in adult lung transplant patients with suspected RVI, compared with serology and culture. Nasopharyngeal and throat swabs (NT) were obtained to sample epithelial cells, followed by application of monoclonal antibody to respiratory syncytial virus, adenovirus, parainfluenza 1–3 and influenza A and B. The Bartels Respiratory Viral Detection kit was used with IFA results available within 24 hours. RESULTS: Nine of 18 patients tested positive for RVI with influenza A (n = 8) and influenza B (n = 1) detected. The sensitivity of IFA (67%) was higher than that of cell culture (45%). With intensive supportive therapy, infection was self-limiting in bronchiolitis obliterans syndrome (BOS) Grade 0–2 patients. However, patients with BOS Grade 3 manifested an acute exacerbation of airflow obstruction, which proved to be irreversible. CONCLUSIONS: Lung transplant patients with “flu-like” symptoms should proceed to IFA testing of NT swab specimens for early diagnosis. Samples collected within 7 days of symptom onset have high sensitivity as compared with serology and viral culture techniques.
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spelling pubmed-71280932020-04-08 Indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients Hopkins, Peter M Plit, Marshall L Carter, Ian W Chhajed, Prashant N Malouf, Monique A Glanville, Allan R J Heart Lung Transplant Article BACKGROUND: Rapid and reliable diagnosis of respiratory viral infections (RVI) in lung transplant recipients is essential to direct therapy of acute graft dysfunction and identify epidemic trends. Traditional techniques of serology and viral culture are limited by the lack of antibody response and delay in diagnosis. METHODS: We examined the clinical utility of indirect fluorescent antibody (IFA) testing in adult lung transplant patients with suspected RVI, compared with serology and culture. Nasopharyngeal and throat swabs (NT) were obtained to sample epithelial cells, followed by application of monoclonal antibody to respiratory syncytial virus, adenovirus, parainfluenza 1–3 and influenza A and B. The Bartels Respiratory Viral Detection kit was used with IFA results available within 24 hours. RESULTS: Nine of 18 patients tested positive for RVI with influenza A (n = 8) and influenza B (n = 1) detected. The sensitivity of IFA (67%) was higher than that of cell culture (45%). With intensive supportive therapy, infection was self-limiting in bronchiolitis obliterans syndrome (BOS) Grade 0–2 patients. However, patients with BOS Grade 3 manifested an acute exacerbation of airflow obstruction, which proved to be irreversible. CONCLUSIONS: Lung transplant patients with “flu-like” symptoms should proceed to IFA testing of NT swab specimens for early diagnosis. Samples collected within 7 days of symptom onset have high sensitivity as compared with serology and viral culture techniques. International Society for Heart and Lung Transplantation. Published by Elsevier Inc. 2003-02 2003-02-05 /pmc/articles/PMC7128093/ /pubmed/12581764 http://dx.doi.org/10.1016/S1053-2498(02)00467-9 Text en Copyright © 2003 International Society for Heart and Lung Transplantation. Published by Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Hopkins, Peter M
Plit, Marshall L
Carter, Ian W
Chhajed, Prashant N
Malouf, Monique A
Glanville, Allan R
Indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients
title Indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients
title_full Indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients
title_fullStr Indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients
title_full_unstemmed Indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients
title_short Indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients
title_sort indirect fluorescent antibody testing of nasopharyngeal swabs for influenza diagnosis in lung transplant recipients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128093/
https://www.ncbi.nlm.nih.gov/pubmed/12581764
http://dx.doi.org/10.1016/S1053-2498(02)00467-9
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