Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex(®) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens

This prospective study was performed to evaluate and compare the performance of the multiplex PCR Seeplex(®) assays and Anyplex™ II assays. From May 2014 until April 2016, a total of 247 respiratory samples were collected in Okinawa, Japan. Multiple respiratory pathogens were detected in 37% of pati...

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Detalles Bibliográficos
Autores principales: Parrott, Gretchen, Kinjo, Takeshi, Nabeya, Daijiro, Uehara, Ayako, Nahar, Saifun, Miyagi, Kazuya, Haranaga, Shusaku, Tateyama, Masao, Fujita, Jiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. 2017
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128259/
https://www.ncbi.nlm.nih.gov/pubmed/28830668
http://dx.doi.org/10.1016/j.jiac.2017.07.013
Descripción
Sumario:This prospective study was performed to evaluate and compare the performance of the multiplex PCR Seeplex(®) assays and Anyplex™ II assays. From May 2014 until April 2016, a total of 247 respiratory samples were collected in Okinawa, Japan. Multiple respiratory pathogens were detected in 37% of patients with positive results. The most prevalent pathogens were influenza A virus and respiratory syncytial virus B. Despite minor differences in capabilities, both the Seeplex(®) assays and Anyplex™ II assays can be easily implemented in diagnostic or research laboratories to optimize the detection and management of respiratory pathogen induced diseases.

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