Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex(®) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens

This prospective study was performed to evaluate and compare the performance of the multiplex PCR Seeplex(®) assays and Anyplex™ II assays. From May 2014 until April 2016, a total of 247 respiratory samples were collected in Okinawa, Japan. Multiple respiratory pathogens were detected in 37% of pati...

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Autores principales: Parrott, Gretchen, Kinjo, Takeshi, Nabeya, Daijiro, Uehara, Ayako, Nahar, Saifun, Miyagi, Kazuya, Haranaga, Shusaku, Tateyama, Masao, Fujita, Jiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. 2017
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128259/
https://www.ncbi.nlm.nih.gov/pubmed/28830668
http://dx.doi.org/10.1016/j.jiac.2017.07.013
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author Parrott, Gretchen
Kinjo, Takeshi
Nabeya, Daijiro
Uehara, Ayako
Nahar, Saifun
Miyagi, Kazuya
Haranaga, Shusaku
Tateyama, Masao
Fujita, Jiro
author_facet Parrott, Gretchen
Kinjo, Takeshi
Nabeya, Daijiro
Uehara, Ayako
Nahar, Saifun
Miyagi, Kazuya
Haranaga, Shusaku
Tateyama, Masao
Fujita, Jiro
author_sort Parrott, Gretchen
collection PubMed
description This prospective study was performed to evaluate and compare the performance of the multiplex PCR Seeplex(®) assays and Anyplex™ II assays. From May 2014 until April 2016, a total of 247 respiratory samples were collected in Okinawa, Japan. Multiple respiratory pathogens were detected in 37% of patients with positive results. The most prevalent pathogens were influenza A virus and respiratory syncytial virus B. Despite minor differences in capabilities, both the Seeplex(®) assays and Anyplex™ II assays can be easily implemented in diagnostic or research laboratories to optimize the detection and management of respiratory pathogen induced diseases.
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spelling pubmed-71282592020-04-08 Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex(®) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens Parrott, Gretchen Kinjo, Takeshi Nabeya, Daijiro Uehara, Ayako Nahar, Saifun Miyagi, Kazuya Haranaga, Shusaku Tateyama, Masao Fujita, Jiro J Infect Chemother Note This prospective study was performed to evaluate and compare the performance of the multiplex PCR Seeplex(®) assays and Anyplex™ II assays. From May 2014 until April 2016, a total of 247 respiratory samples were collected in Okinawa, Japan. Multiple respiratory pathogens were detected in 37% of patients with positive results. The most prevalent pathogens were influenza A virus and respiratory syncytial virus B. Despite minor differences in capabilities, both the Seeplex(®) assays and Anyplex™ II assays can be easily implemented in diagnostic or research laboratories to optimize the detection and management of respiratory pathogen induced diseases. Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. 2017-12 2017-08-19 /pmc/articles/PMC7128259/ /pubmed/28830668 http://dx.doi.org/10.1016/j.jiac.2017.07.013 Text en © 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Note
Parrott, Gretchen
Kinjo, Takeshi
Nabeya, Daijiro
Uehara, Ayako
Nahar, Saifun
Miyagi, Kazuya
Haranaga, Shusaku
Tateyama, Masao
Fujita, Jiro
Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex(®) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens
title Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex(®) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens
title_full Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex(®) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens
title_fullStr Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex(®) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens
title_full_unstemmed Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex(®) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens
title_short Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex(®) RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens
title_sort evaluation of anyplex™ ii rv16 and rb5 real-time rt-pcr compared to seeplex(®) rv15 onestep ace and pneumobacter ace for the simultaneous detection of upper respiratory pathogens
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128259/
https://www.ncbi.nlm.nih.gov/pubmed/28830668
http://dx.doi.org/10.1016/j.jiac.2017.07.013
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