Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus

OBJECTIVE: To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. METHODS: We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time...

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Detalles Bibliográficos
Autores principales: Pongsiri, Piyathida, Praianantathavorn, Kesmanee, Theamboonlers, Apiradee, Payungporn, Sunchai, Poovorawan, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hainan Medical College. Published by Elsevier (Singapore) Pte Ltd. 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128799/
https://www.ncbi.nlm.nih.gov/pubmed/22546647
http://dx.doi.org/10.1016/S1995-7645(12)60055-8
Descripción
Sumario:OBJECTIVE: To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. METHODS: We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR. RESULTS: The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus. CONCLUSIONS: This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.

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