Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus

OBJECTIVE: To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. METHODS: We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time...

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Autores principales: Pongsiri, Piyathida, Praianantathavorn, Kesmanee, Theamboonlers, Apiradee, Payungporn, Sunchai, Poovorawan, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hainan Medical College. Published by Elsevier (Singapore) Pte Ltd. 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128799/
https://www.ncbi.nlm.nih.gov/pubmed/22546647
http://dx.doi.org/10.1016/S1995-7645(12)60055-8
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author Pongsiri, Piyathida
Praianantathavorn, Kesmanee
Theamboonlers, Apiradee
Payungporn, Sunchai
Poovorawan, Yong
author_facet Pongsiri, Piyathida
Praianantathavorn, Kesmanee
Theamboonlers, Apiradee
Payungporn, Sunchai
Poovorawan, Yong
author_sort Pongsiri, Piyathida
collection PubMed
description OBJECTIVE: To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. METHODS: We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR. RESULTS: The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus. CONCLUSIONS: This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.
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spelling pubmed-71287992020-04-08 Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus Pongsiri, Piyathida Praianantathavorn, Kesmanee Theamboonlers, Apiradee Payungporn, Sunchai Poovorawan, Yong Asian Pac J Trop Med Article OBJECTIVE: To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection. METHODS: We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR. RESULTS: The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus. CONCLUSIONS: This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction. Hainan Medical College. Published by Elsevier (Singapore) Pte Ltd. 2012-05 2012-04-28 /pmc/articles/PMC7128799/ /pubmed/22546647 http://dx.doi.org/10.1016/S1995-7645(12)60055-8 Text en Copyright © 2012 Hainan Medical College. Published by Elsevier (Singapore) Pte Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Pongsiri, Piyathida
Praianantathavorn, Kesmanee
Theamboonlers, Apiradee
Payungporn, Sunchai
Poovorawan, Yong
Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus
title Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus
title_full Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus
title_fullStr Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus
title_full_unstemmed Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus
title_short Multiplex real–time RT–PCR for detecting chikungunya virus and dengue virus
title_sort multiplex real–time rt–pcr for detecting chikungunya virus and dengue virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128799/
https://www.ncbi.nlm.nih.gov/pubmed/22546647
http://dx.doi.org/10.1016/S1995-7645(12)60055-8
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