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Enhancing ergosterol production in Pichia pastoris GS115 by overexpressing squalene synthase gene from Glycyrrhiza uralensis
The present study was designed to determine the effects of copy number variations (CNVs) of squalene synthase 1(SQS1) gene on the mevalonate (MVA) pathway. SQS1 gene from G. uralensis (GuSQS1) was cloned and over-expressed in Pichia pastoris GS115. Six recombinant P. pastoris strains containing diff...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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China Pharmaceutical University. Published by Elsevier B.V.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128851/ https://www.ncbi.nlm.nih.gov/pubmed/25986282 http://dx.doi.org/10.1016/S1875-5364(15)30024-8 |
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author | LIU, Ying ZHU, Xiao-Qing LI, Wen-Dong WEN, Hao LIU, Chun-Sheng |
author_facet | LIU, Ying ZHU, Xiao-Qing LI, Wen-Dong WEN, Hao LIU, Chun-Sheng |
author_sort | LIU, Ying |
collection | PubMed |
description | The present study was designed to determine the effects of copy number variations (CNVs) of squalene synthase 1(SQS1) gene on the mevalonate (MVA) pathway. SQS1 gene from G. uralensis (GuSQS1) was cloned and over-expressed in Pichia pastoris GS115. Six recombinant P. pastoris strains containing different copy number of GuSQS1 were constructed. HPLC was used to assay the level of ergosterol in all transgenic P. pastoris strains containing GuSQS1. HPLC analysis showed that the contents of ergosterol in all of the transgenic P. pastoris containing GuSQS1 were higher than that in the negative control. And with the increase of copy number of GuSQS1, the content of ergosterol showed an increasing-decreasing-increasing pattern. The contents of ergosterol in 10-copy-GuSQS1 P. pastoris and 47-copy-GuSQS1 P. pastoris were significantly higher than that in the rest recombinant P. pastoris strains. In conclusion, the CNVs of GuSQS1 influence the content of secondary metabolites in the MVA pathway. The present study provides a basis for over-expressing GuSQS1 and increasing the content of glycyrrhizin in G. uralensis cultivars. |
format | Online Article Text |
id | pubmed-7128851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | China Pharmaceutical University. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71288512020-04-08 Enhancing ergosterol production in Pichia pastoris GS115 by overexpressing squalene synthase gene from Glycyrrhiza uralensis LIU, Ying ZHU, Xiao-Qing LI, Wen-Dong WEN, Hao LIU, Chun-Sheng Chin J Nat Med Articles The present study was designed to determine the effects of copy number variations (CNVs) of squalene synthase 1(SQS1) gene on the mevalonate (MVA) pathway. SQS1 gene from G. uralensis (GuSQS1) was cloned and over-expressed in Pichia pastoris GS115. Six recombinant P. pastoris strains containing different copy number of GuSQS1 were constructed. HPLC was used to assay the level of ergosterol in all transgenic P. pastoris strains containing GuSQS1. HPLC analysis showed that the contents of ergosterol in all of the transgenic P. pastoris containing GuSQS1 were higher than that in the negative control. And with the increase of copy number of GuSQS1, the content of ergosterol showed an increasing-decreasing-increasing pattern. The contents of ergosterol in 10-copy-GuSQS1 P. pastoris and 47-copy-GuSQS1 P. pastoris were significantly higher than that in the rest recombinant P. pastoris strains. In conclusion, the CNVs of GuSQS1 influence the content of secondary metabolites in the MVA pathway. The present study provides a basis for over-expressing GuSQS1 and increasing the content of glycyrrhizin in G. uralensis cultivars. China Pharmaceutical University. Published by Elsevier B.V. 2015-05 2015-05-17 /pmc/articles/PMC7128851/ /pubmed/25986282 http://dx.doi.org/10.1016/S1875-5364(15)30024-8 Text en © 2015 China Pharmaceutical University Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Articles LIU, Ying ZHU, Xiao-Qing LI, Wen-Dong WEN, Hao LIU, Chun-Sheng Enhancing ergosterol production in Pichia pastoris GS115 by overexpressing squalene synthase gene from Glycyrrhiza uralensis |
title | Enhancing ergosterol production in Pichia pastoris GS115 by overexpressing squalene synthase gene from Glycyrrhiza uralensis |
title_full | Enhancing ergosterol production in Pichia pastoris GS115 by overexpressing squalene synthase gene from Glycyrrhiza uralensis |
title_fullStr | Enhancing ergosterol production in Pichia pastoris GS115 by overexpressing squalene synthase gene from Glycyrrhiza uralensis |
title_full_unstemmed | Enhancing ergosterol production in Pichia pastoris GS115 by overexpressing squalene synthase gene from Glycyrrhiza uralensis |
title_short | Enhancing ergosterol production in Pichia pastoris GS115 by overexpressing squalene synthase gene from Glycyrrhiza uralensis |
title_sort | enhancing ergosterol production in pichia pastoris gs115 by overexpressing squalene synthase gene from glycyrrhiza uralensis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128851/ https://www.ncbi.nlm.nih.gov/pubmed/25986282 http://dx.doi.org/10.1016/S1875-5364(15)30024-8 |
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