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Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests
An ongoing outbreak of severe respiratory pneumonia associated with the 2019 novel coronavirus has recently emerged in China. Here we report the epidemiological, clinical, laboratory and radiological characteristics of 19 suspect cases. We compared the positive ratio of 2019-nCoV nucleic acid amplif...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
University of Electronic Science and Technology of China, Chengdu, China. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129110/ https://www.ncbi.nlm.nih.gov/pubmed/32114193 http://dx.doi.org/10.1016/j.ijid.2020.02.050 |
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author | Xie, Chunbao Jiang, Lingxi Huang, Guo Pu, Hong Gong, Bo Lin, He Ma, Shi Chen, Xuemei Long, Bo Si, Guo Yu, Hua Jiang, Li Yang, Xingxiang Shi, Yi Yang, Zhenglin |
author_facet | Xie, Chunbao Jiang, Lingxi Huang, Guo Pu, Hong Gong, Bo Lin, He Ma, Shi Chen, Xuemei Long, Bo Si, Guo Yu, Hua Jiang, Li Yang, Xingxiang Shi, Yi Yang, Zhenglin |
author_sort | Xie, Chunbao |
collection | PubMed |
description | An ongoing outbreak of severe respiratory pneumonia associated with the 2019 novel coronavirus has recently emerged in China. Here we report the epidemiological, clinical, laboratory and radiological characteristics of 19 suspect cases. We compared the positive ratio of 2019-nCoV nucleic acid amplification test results from different samples including oropharyngeal swab, blood, urine and stool with 3 different fluorescent RT-PCR kits. Nine out of the 19 patients had 2019-nCoV infection detected using oropharyngeal swab samples, and the virus nucleic acid was also detected in eight of these nine patients using stool samples. None of positive results was identified in the blood and urine samples. These three different kits got the same result for each sample and the positive ratio of nucleic acid detection for 2019-nCoV was only 47.4% in the suspect patients. Therefore, it is possible that infected patients have been missed by using nucleic acid detection only. It might be better to make a diagnosis combining the computed tomography scans and nucleic acid detection. |
format | Online Article Text |
id | pubmed-7129110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | University of Electronic Science and Technology of China, Chengdu, China. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71291102020-04-08 Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests Xie, Chunbao Jiang, Lingxi Huang, Guo Pu, Hong Gong, Bo Lin, He Ma, Shi Chen, Xuemei Long, Bo Si, Guo Yu, Hua Jiang, Li Yang, Xingxiang Shi, Yi Yang, Zhenglin Int J Infect Dis Article An ongoing outbreak of severe respiratory pneumonia associated with the 2019 novel coronavirus has recently emerged in China. Here we report the epidemiological, clinical, laboratory and radiological characteristics of 19 suspect cases. We compared the positive ratio of 2019-nCoV nucleic acid amplification test results from different samples including oropharyngeal swab, blood, urine and stool with 3 different fluorescent RT-PCR kits. Nine out of the 19 patients had 2019-nCoV infection detected using oropharyngeal swab samples, and the virus nucleic acid was also detected in eight of these nine patients using stool samples. None of positive results was identified in the blood and urine samples. These three different kits got the same result for each sample and the positive ratio of nucleic acid detection for 2019-nCoV was only 47.4% in the suspect patients. Therefore, it is possible that infected patients have been missed by using nucleic acid detection only. It might be better to make a diagnosis combining the computed tomography scans and nucleic acid detection. University of Electronic Science and Technology of China, Chengdu, China. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2020-04 2020-02-27 /pmc/articles/PMC7129110/ /pubmed/32114193 http://dx.doi.org/10.1016/j.ijid.2020.02.050 Text en © 2020 University of Electronic Science and Technology of China, Chengdu, China Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Xie, Chunbao Jiang, Lingxi Huang, Guo Pu, Hong Gong, Bo Lin, He Ma, Shi Chen, Xuemei Long, Bo Si, Guo Yu, Hua Jiang, Li Yang, Xingxiang Shi, Yi Yang, Zhenglin Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests |
title | Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests |
title_full | Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests |
title_fullStr | Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests |
title_full_unstemmed | Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests |
title_short | Comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests |
title_sort | comparison of different samples for 2019 novel coronavirus detection by nucleic acid amplification tests |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129110/ https://www.ncbi.nlm.nih.gov/pubmed/32114193 http://dx.doi.org/10.1016/j.ijid.2020.02.050 |
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