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Association between faecal shedding of feline coronavirus and serum α1-acid glycoprotein sialylation

The sialylation pattern of serum α1-acid glycoprotein (AGP) in non-symptomatic cats infected by feline coronavirus (FCoV) and its possible relationship with the amount of FCoVs shed in faeces were investigated. Blood from three specific pathogen-free cats (group A) and from 10 non-symptomatic FCoV-p...

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Autores principales: Paltrinieri, Saverio, Gelain, Maria E., Ceciliani, Fabrizio, Ribera, Alba M., Battilani, Mara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: ESFM and AAFP. Published by Elsevier Ltd. 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129531/
https://www.ncbi.nlm.nih.gov/pubmed/18701332
http://dx.doi.org/10.1016/j.jfms.2008.04.004
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author Paltrinieri, Saverio
Gelain, Maria E.
Ceciliani, Fabrizio
Ribera, Alba M.
Battilani, Mara
author_facet Paltrinieri, Saverio
Gelain, Maria E.
Ceciliani, Fabrizio
Ribera, Alba M.
Battilani, Mara
author_sort Paltrinieri, Saverio
collection PubMed
description The sialylation pattern of serum α1-acid glycoprotein (AGP) in non-symptomatic cats infected by feline coronavirus (FCoV) and its possible relationship with the amount of FCoVs shed in faeces were investigated. Blood from three specific pathogen-free cats (group A) and from 10 non-symptomatic FCoV-positive cats from catteries with low (group B, three cats) or high (group C, seven cats) levels of faecal shedding were collected monthly. AGP was purified from serum and Western blotting followed by lectin-staining of α(2,3)-linked and α(2,6)-linked sialic acid. Faecal shedding was quantified in group C by quantitative polymerase chain reaction. Variations of AGP sialylation were recorded only in cats from group C, on which viral shedding peaked before the occurrence of feline infectious peritonitis (FIP) in the cattery, and decreased 1 month later, when serum AGP had an increase of α(2,3)-linked sialic acid. These results suggest that hypersialylation of AGP may be involved in host–virus interactions.
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spelling pubmed-71295312020-04-08 Association between faecal shedding of feline coronavirus and serum α1-acid glycoprotein sialylation Paltrinieri, Saverio Gelain, Maria E. Ceciliani, Fabrizio Ribera, Alba M. Battilani, Mara J Feline Med Surg Short Communication The sialylation pattern of serum α1-acid glycoprotein (AGP) in non-symptomatic cats infected by feline coronavirus (FCoV) and its possible relationship with the amount of FCoVs shed in faeces were investigated. Blood from three specific pathogen-free cats (group A) and from 10 non-symptomatic FCoV-positive cats from catteries with low (group B, three cats) or high (group C, seven cats) levels of faecal shedding were collected monthly. AGP was purified from serum and Western blotting followed by lectin-staining of α(2,3)-linked and α(2,6)-linked sialic acid. Faecal shedding was quantified in group C by quantitative polymerase chain reaction. Variations of AGP sialylation were recorded only in cats from group C, on which viral shedding peaked before the occurrence of feline infectious peritonitis (FIP) in the cattery, and decreased 1 month later, when serum AGP had an increase of α(2,3)-linked sialic acid. These results suggest that hypersialylation of AGP may be involved in host–virus interactions. ESFM and AAFP. Published by Elsevier Ltd. 2008-10 2008-08-12 /pmc/articles/PMC7129531/ /pubmed/18701332 http://dx.doi.org/10.1016/j.jfms.2008.04.004 Text en Copyright © 2008 ESFM and AAFP. Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Paltrinieri, Saverio
Gelain, Maria E.
Ceciliani, Fabrizio
Ribera, Alba M.
Battilani, Mara
Association between faecal shedding of feline coronavirus and serum α1-acid glycoprotein sialylation
title Association between faecal shedding of feline coronavirus and serum α1-acid glycoprotein sialylation
title_full Association between faecal shedding of feline coronavirus and serum α1-acid glycoprotein sialylation
title_fullStr Association between faecal shedding of feline coronavirus and serum α1-acid glycoprotein sialylation
title_full_unstemmed Association between faecal shedding of feline coronavirus and serum α1-acid glycoprotein sialylation
title_short Association between faecal shedding of feline coronavirus and serum α1-acid glycoprotein sialylation
title_sort association between faecal shedding of feline coronavirus and serum α1-acid glycoprotein sialylation
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129531/
https://www.ncbi.nlm.nih.gov/pubmed/18701332
http://dx.doi.org/10.1016/j.jfms.2008.04.004
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