Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chi...

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Detalles Bibliográficos
Autores principales: Hacker, David L., Kiseljak, Divor, Rajendra, Yashas, Thurnheer, Sarah, Baldi, Lucia, Wurm, Florian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2013
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129890/
https://www.ncbi.nlm.nih.gov/pubmed/24021764
http://dx.doi.org/10.1016/j.pep.2013.09.001
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author Hacker, David L.
Kiseljak, Divor
Rajendra, Yashas
Thurnheer, Sarah
Baldi, Lucia
Wurm, Florian M.
author_facet Hacker, David L.
Kiseljak, Divor
Rajendra, Yashas
Thurnheer, Sarah
Baldi, Lucia
Wurm, Florian M.
author_sort Hacker, David L.
collection PubMed
description Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery.
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spelling pubmed-71298902020-04-08 Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells Hacker, David L. Kiseljak, Divor Rajendra, Yashas Thurnheer, Sarah Baldi, Lucia Wurm, Florian M. Protein Expr Purif Review Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery. Elsevier Inc. 2013-11 2013-09-07 /pmc/articles/PMC7129890/ /pubmed/24021764 http://dx.doi.org/10.1016/j.pep.2013.09.001 Text en Copyright © 2013 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Review
Hacker, David L.
Kiseljak, Divor
Rajendra, Yashas
Thurnheer, Sarah
Baldi, Lucia
Wurm, Florian M.
Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
title Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
title_full Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
title_fullStr Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
title_full_unstemmed Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
title_short Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
title_sort polyethyleneimine-based transient gene expression processes for suspension-adapted hek-293e and cho-dg44 cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129890/
https://www.ncbi.nlm.nih.gov/pubmed/24021764
http://dx.doi.org/10.1016/j.pep.2013.09.001
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