Prokaryotic expression, refolding, and purification of fragment 450–650 of the spike protein of SARS-coronavirus

The spike (S) glycoprotein is one of the major structure proteins of SARS-associated coronavirus (CoV). Fragment 450–650 (S450-650) of the S protein contains receptor-binding domain and neutralizing epitopes. In this study, S450-650 was expressed with a histidine tag in Escherichia coli BL21. Bacter...

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Detalles Bibliográficos
Autores principales: Zhao, Jin-Cun, Zhao, Zhen-Dong, Wang, Wei, Gao, Xiao-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129930/
https://www.ncbi.nlm.nih.gov/pubmed/15642467
http://dx.doi.org/10.1016/j.pep.2004.10.004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129930/
https://www.ncbi.nlm.nih.gov/pubmed/15642467
http://dx.doi.org/10.1016/j.pep.2004.10.004

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