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Prokaryotic expression, refolding, and purification of fragment 450–650 of the spike protein of SARS-coronavirus
The spike (S) glycoprotein is one of the major structure proteins of SARS-associated coronavirus (CoV). Fragment 450–650 (S450-650) of the S protein contains receptor-binding domain and neutralizing epitopes. In this study, S450-650 was expressed with a histidine tag in Escherichia coli BL21. Bacter...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Inc.
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129930/ https://www.ncbi.nlm.nih.gov/pubmed/15642467 http://dx.doi.org/10.1016/j.pep.2004.10.004 |