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The oligomeric structure of renal aminopeptidase N from bovine brush-border membrane vesicles

Bovine renal brush-border membrane vesicle aminopeptidase N at various stages of purity was treated with two bifunctional cross-linking agents. A pattern of emergence of higher molecular weight forms was observed. By using a cleavable cross-linker, aminopeptidase N was shown to cross-link both to it...

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Detalles Bibliográficos
Autores principales: Plakidou-Dymock, Stella, McGivan, John D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130128/
https://www.ncbi.nlm.nih.gov/pubmed/8093665
http://dx.doi.org/10.1016/0005-2736(93)90386-E
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author Plakidou-Dymock, Stella
McGivan, John D.
author_facet Plakidou-Dymock, Stella
McGivan, John D.
author_sort Plakidou-Dymock, Stella
collection PubMed
description Bovine renal brush-border membrane vesicle aminopeptidase N at various stages of purity was treated with two bifunctional cross-linking agents. A pattern of emergence of higher molecular weight forms was observed. By using a cleavable cross-linker, aminopeptidase N was shown to cross-link both to itself and to its breakdown products as well as to dipeptidyl peptidase IV. Using this technique it was possible to identify three of the breakdown products as 45 kDa, 66 kDa and 95 kDa peptides. N-terminal amino acid sequence analysis was used to define the precise cleavage points for the bovine renal aminopeptidase N breakdown products. The short amino acid sequences obtained show strong sequence similarity with the human intestinal and rat kidney aminopeptidase N.
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spelling pubmed-71301282020-04-08 The oligomeric structure of renal aminopeptidase N from bovine brush-border membrane vesicles Plakidou-Dymock, Stella McGivan, John D. Biochim Biophys Acta Biomembr Article Bovine renal brush-border membrane vesicle aminopeptidase N at various stages of purity was treated with two bifunctional cross-linking agents. A pattern of emergence of higher molecular weight forms was observed. By using a cleavable cross-linker, aminopeptidase N was shown to cross-link both to itself and to its breakdown products as well as to dipeptidyl peptidase IV. Using this technique it was possible to identify three of the breakdown products as 45 kDa, 66 kDa and 95 kDa peptides. N-terminal amino acid sequence analysis was used to define the precise cleavage points for the bovine renal aminopeptidase N breakdown products. The short amino acid sequences obtained show strong sequence similarity with the human intestinal and rat kidney aminopeptidase N. Published by Elsevier B.V. 1993-01-18 2003-02-03 /pmc/articles/PMC7130128/ /pubmed/8093665 http://dx.doi.org/10.1016/0005-2736(93)90386-E Text en Copyright © 1993 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Plakidou-Dymock, Stella
McGivan, John D.
The oligomeric structure of renal aminopeptidase N from bovine brush-border membrane vesicles
title The oligomeric structure of renal aminopeptidase N from bovine brush-border membrane vesicles
title_full The oligomeric structure of renal aminopeptidase N from bovine brush-border membrane vesicles
title_fullStr The oligomeric structure of renal aminopeptidase N from bovine brush-border membrane vesicles
title_full_unstemmed The oligomeric structure of renal aminopeptidase N from bovine brush-border membrane vesicles
title_short The oligomeric structure of renal aminopeptidase N from bovine brush-border membrane vesicles
title_sort oligomeric structure of renal aminopeptidase n from bovine brush-border membrane vesicles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130128/
https://www.ncbi.nlm.nih.gov/pubmed/8093665
http://dx.doi.org/10.1016/0005-2736(93)90386-E
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