Acidic pH induces fusion of cells infected with baculovirus to form syncytia

The enveloped baculovirus⧹insect cell system has been used extensively for expression of recombinant proteins. including viral fusion proteins. We tested wild‐type baculovirus for endogenous fusion protein activity. Syncytia formation, dye transfer, and capacitance changes were observed after incuba...

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Detalles Bibliográficos
Autores principales: Leikina, Evgenia, Onaran, H.Ongun, Zimmerberg, Joshua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 1992
Materias:
Full‐length Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130246/
https://www.ncbi.nlm.nih.gov/pubmed/1618326
http://dx.doi.org/10.1016/0014-5793(92)80623-O
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author Leikina, Evgenia
Onaran, H.Ongun
Zimmerberg, Joshua
author_facet Leikina, Evgenia
Onaran, H.Ongun
Zimmerberg, Joshua
author_sort Leikina, Evgenia
collection PubMed
description The enveloped baculovirus⧹insect cell system has been used extensively for expression of recombinant proteins. including viral fusion proteins. We tested wild‐type baculovirus for endogenous fusion protein activity. Syncytia formation, dye transfer, and capacitance changes were observed after incubating infected Spodoptera frugiperda cells in acidic media, consistent with fusion protein activity. Only a short acidic pulse or 10 s is needed to trigger syncytia formation. Identical results were obtained with recombinant baculovirus. This new system convenient for studying pH activated cell‐cell fusion. However, using this enveloped virus to study the mechanism of recombinant fusion proteins requires caution.
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spelling pubmed-71302462020-04-08 Acidic pH induces fusion of cells infected with baculovirus to form syncytia Leikina, Evgenia Onaran, H.Ongun Zimmerberg, Joshua FEBS Lett Full‐length Articles The enveloped baculovirus⧹insect cell system has been used extensively for expression of recombinant proteins. including viral fusion proteins. We tested wild‐type baculovirus for endogenous fusion protein activity. Syncytia formation, dye transfer, and capacitance changes were observed after incubating infected Spodoptera frugiperda cells in acidic media, consistent with fusion protein activity. Only a short acidic pulse or 10 s is needed to trigger syncytia formation. Identical results were obtained with recombinant baculovirus. This new system convenient for studying pH activated cell‐cell fusion. However, using this enveloped virus to study the mechanism of recombinant fusion proteins requires caution. John Wiley and Sons Inc. 1992-06-15 2002-01-16 /pmc/articles/PMC7130246/ /pubmed/1618326 http://dx.doi.org/10.1016/0014-5793(92)80623-O Text en FEBS Letters 304 (1992) 1873-3468 © 2015 Federation of European Biochemical Societies This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.
spellingShingle Full‐length Articles
Leikina, Evgenia
Onaran, H.Ongun
Zimmerberg, Joshua
Acidic pH induces fusion of cells infected with baculovirus to form syncytia
title Acidic pH induces fusion of cells infected with baculovirus to form syncytia
title_full Acidic pH induces fusion of cells infected with baculovirus to form syncytia
title_fullStr Acidic pH induces fusion of cells infected with baculovirus to form syncytia
title_full_unstemmed Acidic pH induces fusion of cells infected with baculovirus to form syncytia
title_short Acidic pH induces fusion of cells infected with baculovirus to form syncytia
title_sort acidic ph induces fusion of cells infected with baculovirus to form syncytia
topic Full‐length Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130246/
https://www.ncbi.nlm.nih.gov/pubmed/1618326
http://dx.doi.org/10.1016/0014-5793(92)80623-O
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AT zimmerbergjoshua acidicphinducesfusionofcellsinfectedwithbaculovirustoformsyncytia

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