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Métodos moleculares de diagnóstico de infecciones respiratorias. ¿Ha cambiado el esquema diagnóstico?
Lower respiratory tract infections remain one of the most common causes of mortality worldwide, which is why early diagnosis is crucial. Traditionally the microbiological diagnosis of these infections has been based on conventional methods including culture on artificial media for isolation of bacte...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier España, S.L.U.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130258/ https://www.ncbi.nlm.nih.gov/pubmed/27474246 http://dx.doi.org/10.1016/S0213-005X(16)30218-X |
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author | Vila Estapé, Jordi Zboromyrska, Yuliya Vergara Gómez, Andrea Alejo Cancho, Izaskun Rubio García, Elisa Álvarez-Martínez, Miriam José la Bellacasa Brugada, Jorge Puig de Marcos Maeso, M. Ángeles |
author_facet | Vila Estapé, Jordi Zboromyrska, Yuliya Vergara Gómez, Andrea Alejo Cancho, Izaskun Rubio García, Elisa Álvarez-Martínez, Miriam José la Bellacasa Brugada, Jorge Puig de Marcos Maeso, M. Ángeles |
author_sort | Vila Estapé, Jordi |
collection | PubMed |
description | Lower respiratory tract infections remain one of the most common causes of mortality worldwide, which is why early diagnosis is crucial. Traditionally the microbiological diagnosis of these infections has been based on conventional methods including culture on artificial media for isolation of bacteria and fungi and cell cultures for virus and antibody or antigen detection using antigen-antibody reactions. The main drawback of the above mentioned methods is the time needed for an etiological diagnosis of the infection. The techniques based on molecular biology have drawn much attention in recent decades as tools for rapid diagnosis of infections. Some techniques are very expensive, especially those that can detect various microorganisms in the same reaction, therefore the question that arises is whether the cost of such testing is justified by the information obtained and by the clinical impact that its implementation will determine. In this article we make a review of the various techniques of molecular biology applied to the diagnosis of pneumonia and focus primarily on analysing the impact they may have on the management of patients with acute respiratory tract infections. |
format | Online Article Text |
id | pubmed-7130258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier España, S.L.U. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71302582020-04-08 Métodos moleculares de diagnóstico de infecciones respiratorias. ¿Ha cambiado el esquema diagnóstico? Vila Estapé, Jordi Zboromyrska, Yuliya Vergara Gómez, Andrea Alejo Cancho, Izaskun Rubio García, Elisa Álvarez-Martínez, Miriam José la Bellacasa Brugada, Jorge Puig de Marcos Maeso, M. Ángeles Enferm Infecc Microbiol Clin Article Lower respiratory tract infections remain one of the most common causes of mortality worldwide, which is why early diagnosis is crucial. Traditionally the microbiological diagnosis of these infections has been based on conventional methods including culture on artificial media for isolation of bacteria and fungi and cell cultures for virus and antibody or antigen detection using antigen-antibody reactions. The main drawback of the above mentioned methods is the time needed for an etiological diagnosis of the infection. The techniques based on molecular biology have drawn much attention in recent decades as tools for rapid diagnosis of infections. Some techniques are very expensive, especially those that can detect various microorganisms in the same reaction, therefore the question that arises is whether the cost of such testing is justified by the information obtained and by the clinical impact that its implementation will determine. In this article we make a review of the various techniques of molecular biology applied to the diagnosis of pneumonia and focus primarily on analysing the impact they may have on the management of patients with acute respiratory tract infections. Elsevier España, S.L.U. 2016-07 2016-07-26 /pmc/articles/PMC7130258/ /pubmed/27474246 http://dx.doi.org/10.1016/S0213-005X(16)30218-X Text en Copyright © 2016 Elsevier España, S.L.U. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Vila Estapé, Jordi Zboromyrska, Yuliya Vergara Gómez, Andrea Alejo Cancho, Izaskun Rubio García, Elisa Álvarez-Martínez, Miriam José la Bellacasa Brugada, Jorge Puig de Marcos Maeso, M. Ángeles Métodos moleculares de diagnóstico de infecciones respiratorias. ¿Ha cambiado el esquema diagnóstico? |
title | Métodos moleculares de diagnóstico de infecciones respiratorias. ¿Ha cambiado el esquema diagnóstico? |
title_full | Métodos moleculares de diagnóstico de infecciones respiratorias. ¿Ha cambiado el esquema diagnóstico? |
title_fullStr | Métodos moleculares de diagnóstico de infecciones respiratorias. ¿Ha cambiado el esquema diagnóstico? |
title_full_unstemmed | Métodos moleculares de diagnóstico de infecciones respiratorias. ¿Ha cambiado el esquema diagnóstico? |
title_short | Métodos moleculares de diagnóstico de infecciones respiratorias. ¿Ha cambiado el esquema diagnóstico? |
title_sort | métodos moleculares de diagnóstico de infecciones respiratorias. ¿ha cambiado el esquema diagnóstico? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130258/ https://www.ncbi.nlm.nih.gov/pubmed/27474246 http://dx.doi.org/10.1016/S0213-005X(16)30218-X |
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