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Electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus
Five gnotobiotic piglets inoculated orally with porcine rotavirus developed an enteric lesion. Electron microscopy of the mucosal epithelium 12 h after inoculation showed that the virus penetrates into the absorptive cells between microvilli, possibly by a pinocytic mechanism. Afterwards, virus part...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Ltd.
1982
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130348/ https://www.ncbi.nlm.nih.gov/pubmed/6296207 http://dx.doi.org/10.1016/0021-9975(82)90011-1 |
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author | Narita, M. Fukusho, A. Shimizu, Y. |
author_facet | Narita, M. Fukusho, A. Shimizu, Y. |
author_sort | Narita, M. |
collection | PubMed |
description | Five gnotobiotic piglets inoculated orally with porcine rotavirus developed an enteric lesion. Electron microscopy of the mucosal epithelium 12 h after inoculation showed that the virus penetrates into the absorptive cells between microvilli, possibly by a pinocytic mechanism. Afterwards, virus particles were most often seen within dilated cisternae of the rough endoplasmic reticulum (RER). These infected cells showed a range of changes, such as disruption of the microvilli, loss of cytoplasmic density and deposition of lipid droplets. Subsequently, most of the epithelial cells were desquamated from the villi. The interaction of virus and intestinal cells thus indicates that rotavirus is pathogenic for the epithelial cells. |
format | Online Article Text |
id | pubmed-7130348 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1982 |
publisher | Published by Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71303482020-04-08 Electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus Narita, M. Fukusho, A. Shimizu, Y. J Comp Pathol Article Five gnotobiotic piglets inoculated orally with porcine rotavirus developed an enteric lesion. Electron microscopy of the mucosal epithelium 12 h after inoculation showed that the virus penetrates into the absorptive cells between microvilli, possibly by a pinocytic mechanism. Afterwards, virus particles were most often seen within dilated cisternae of the rough endoplasmic reticulum (RER). These infected cells showed a range of changes, such as disruption of the microvilli, loss of cytoplasmic density and deposition of lipid droplets. Subsequently, most of the epithelial cells were desquamated from the villi. The interaction of virus and intestinal cells thus indicates that rotavirus is pathogenic for the epithelial cells. Published by Elsevier Ltd. 1982-10 2004-09-23 /pmc/articles/PMC7130348/ /pubmed/6296207 http://dx.doi.org/10.1016/0021-9975(82)90011-1 Text en Copyright © 1982 Published by Elsevier Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Narita, M. Fukusho, A. Shimizu, Y. Electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus |
title | Electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus |
title_full | Electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus |
title_fullStr | Electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus |
title_full_unstemmed | Electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus |
title_short | Electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus |
title_sort | electron microscopy of the intestine of gnotobiotic piglets infected with porcine rotavirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130348/ https://www.ncbi.nlm.nih.gov/pubmed/6296207 http://dx.doi.org/10.1016/0021-9975(82)90011-1 |
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