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Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet
We studied the macromolecular permeability of segments of jejunum from 2-wk-old piglets after the animals had been experimentally infected with an invasive enteric virus, transmissible gastroenteritis virus. Jejunal segments were mounted in Ussing chambers at stages of the infection, and permeabilit...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Gastroenterological Association. Published by Elsevier Inc.
1985
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130366/ https://www.ncbi.nlm.nih.gov/pubmed/3918915 http://dx.doi.org/10.1016/S0016-5085(85)80020-2 |
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author | Keljo, David J. Butler, Daniel G. Hamilton, J. Richard |
author_facet | Keljo, David J. Butler, Daniel G. Hamilton, J. Richard |
author_sort | Keljo, David J. |
collection | PubMed |
description | We studied the macromolecular permeability of segments of jejunum from 2-wk-old piglets after the animals had been experimentally infected with an invasive enteric virus, transmissible gastroenteritis virus. Jejunal segments were mounted in Ussing chambers at stages of the infection, and permeability was measured using three probe molecules of differing molecular weights. In control tissue, permeability to horseradish peroxidase was 2.6 times higher across segments with Peyer's patches than across segments without Peyer's patches, whereas polyethylene glycol 4000 and mannitol permeabilities were the same in patch and nonpatch segments. Twelve hours after infection, when virus had invaded the mucosa causing a structural lesion, and before diarrhea had begun, horseradish peroxidase permeability increased in non-patch-containing segments to equal that across patch-containing tissue. At this early 12-h stage, polyethylene glycol 4000 and mannitol permeation were unchanged in patchcontaining segments compared with controls. Ninety-six hours after transmissible gastroenteritis infection, when diarrhea was severe, horseradish peroxidase permeability in patch-free segments had returned to normal and patch-containing tissue permeability was diminished below control levels. Increased macromolecular permeability appears to occur only in the very early invasive stage of this viral enteritis and only in patch-free segments. Any consideration of the immunologic relevance of these complex phenomena must take into account the specialized function of the Peyer's patch regions of the small intestine. |
format | Online Article Text |
id | pubmed-7130366 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1985 |
publisher | American Gastroenterological Association. Published by Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71303662020-04-08 Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet Keljo, David J. Butler, Daniel G. Hamilton, J. Richard Gastroenterology Article We studied the macromolecular permeability of segments of jejunum from 2-wk-old piglets after the animals had been experimentally infected with an invasive enteric virus, transmissible gastroenteritis virus. Jejunal segments were mounted in Ussing chambers at stages of the infection, and permeability was measured using three probe molecules of differing molecular weights. In control tissue, permeability to horseradish peroxidase was 2.6 times higher across segments with Peyer's patches than across segments without Peyer's patches, whereas polyethylene glycol 4000 and mannitol permeabilities were the same in patch and nonpatch segments. Twelve hours after infection, when virus had invaded the mucosa causing a structural lesion, and before diarrhea had begun, horseradish peroxidase permeability increased in non-patch-containing segments to equal that across patch-containing tissue. At this early 12-h stage, polyethylene glycol 4000 and mannitol permeation were unchanged in patchcontaining segments compared with controls. Ninety-six hours after transmissible gastroenteritis infection, when diarrhea was severe, horseradish peroxidase permeability in patch-free segments had returned to normal and patch-containing tissue permeability was diminished below control levels. Increased macromolecular permeability appears to occur only in the very early invasive stage of this viral enteritis and only in patch-free segments. Any consideration of the immunologic relevance of these complex phenomena must take into account the specialized function of the Peyer's patch regions of the small intestine. American Gastroenterological Association. Published by Elsevier Inc. 1985-04 2016-08-12 /pmc/articles/PMC7130366/ /pubmed/3918915 http://dx.doi.org/10.1016/S0016-5085(85)80020-2 Text en © 1985 American Gastroenterological Association Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Keljo, David J. Butler, Daniel G. Hamilton, J. Richard Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet |
title | Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet |
title_full | Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet |
title_fullStr | Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet |
title_full_unstemmed | Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet |
title_short | Altered Jejunal Permeability to Macromolecules During Viral Enteritis in the Piglet |
title_sort | altered jejunal permeability to macromolecules during viral enteritis in the piglet |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130366/ https://www.ncbi.nlm.nih.gov/pubmed/3918915 http://dx.doi.org/10.1016/S0016-5085(85)80020-2 |
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