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Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus
The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its “5′ unique region” two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Inc.
1992
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130825/ https://www.ncbi.nlm.nih.gov/pubmed/1309280 http://dx.doi.org/10.1016/0042-6822(92)90094-6 |
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author | Liu, D.X. Inglis, S.C. |
author_facet | Liu, D.X. Inglis, S.C. |
author_sort | Liu, D.X. |
collection | PubMed |
description | The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its “5′ unique region” two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two polypeptides with the sizes expected for the 5a and 5b products can be synthesised by in vitro translation of a single artificial mRNA containing both the 5a and 5b ORFs. To establish whether these polypeptides represent genuine virus gene products, both the 5a and 5b coding sequences were expressed as bacterial fusion proteins, and these were used to raise monospecific antisera. Antisera raised against both the 5a and 5b-specific sequences recognized specifically proteins of the expected size in infectious bronchitis virus-infected chicken kidney and Vero cells, indicating that 5a and 5b do represent genuine virus genes, and suggesting that mRNA5 is indeed functionally dicistronic. |
format | Online Article Text |
id | pubmed-7130825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1992 |
publisher | Published by Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71308252020-04-08 Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus Liu, D.X. Inglis, S.C. Virology Article The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its “5′ unique region” two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two polypeptides with the sizes expected for the 5a and 5b products can be synthesised by in vitro translation of a single artificial mRNA containing both the 5a and 5b ORFs. To establish whether these polypeptides represent genuine virus gene products, both the 5a and 5b coding sequences were expressed as bacterial fusion proteins, and these were used to raise monospecific antisera. Antisera raised against both the 5a and 5b-specific sequences recognized specifically proteins of the expected size in infectious bronchitis virus-infected chicken kidney and Vero cells, indicating that 5a and 5b do represent genuine virus genes, and suggesting that mRNA5 is indeed functionally dicistronic. Published by Elsevier Inc. 1992-01 2004-02-06 /pmc/articles/PMC7130825/ /pubmed/1309280 http://dx.doi.org/10.1016/0042-6822(92)90094-6 Text en Copyright © 1992 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Liu, D.X. Inglis, S.C. Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus |
title | Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus |
title_full | Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus |
title_fullStr | Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus |
title_full_unstemmed | Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus |
title_short | Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus |
title_sort | identification of two new polypeptides encoded by mrna5 of the coronavirus infectious bronchitis virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130825/ https://www.ncbi.nlm.nih.gov/pubmed/1309280 http://dx.doi.org/10.1016/0042-6822(92)90094-6 |
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