Role of oligosaccharides in the structure and function of respiratory syncytial virus glycoproteins

The contribution of oligosaccharides to the structural and functional make-up of respiratory syncytial (RS) virus G and F proteins was investigated by observing the effects of various oligosaccharide-specific enzymes on their molecular size as well as on virus infectivity. The N-linked oligosaccharides of the F protein were completely removed by endoglycosidase F and N-glycanase. Addition of oligosaccharides to F protein during synthesis was completely partially resistant to TM resulting in an 80-kDa form designated G(TM). The G protein was estimated to contain approximately 3% N-linked and 55% O-linked carbohydrates, based on migration of G and G(TM) in polyacrylamide gels. Furthermore, treatment of detergent-extracted G protein with endoglycosidase F and endo-α-N-acetylgalactosaminidase, enzymes that specifically cleave N-linked and O-linked oligosaccharides, respectively, generated a variety of partially unglycosylated species, ranging in molecular weight from approximately 80 to 40 kDa. Virus infectivity was sensitive to limited removal of N-linked or O-linked oligosaccharides by endoglycosidases under conditions which did not greatly alter the molecular weight of the G protein. Thus, G and F protein oligosaccharides readily accessible to enzymatic removal are presumed to play an important role in the infectious process.