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Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion

Mouse hepatitis virus, a coronavirus, was rescued from latently infected mouse neuroblastoma cells by polyethylene glycol-induced fusion to permissive cells. The isolated viruses grew to equal or higher titers than parental JHM virus at 39° but were restricted in replication at 32° and hence were co...

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Autores principales: Stohlman, Stephen A., Sakaguchi, Alan Y., Weiner, Leslie P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 1979
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130992/
https://www.ncbi.nlm.nih.gov/pubmed/228481
http://dx.doi.org/10.1016/0042-6822(79)90567-1
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author Stohlman, Stephen A.
Sakaguchi, Alan Y.
Weiner, Leslie P.
author_facet Stohlman, Stephen A.
Sakaguchi, Alan Y.
Weiner, Leslie P.
author_sort Stohlman, Stephen A.
collection PubMed
description Mouse hepatitis virus, a coronavirus, was rescued from latently infected mouse neuroblastoma cells by polyethylene glycol-induced fusion to permissive cells. The isolated viruses grew to equal or higher titers than parental JHM virus at 39° but were restricted in replication at 32° and hence were cold-sensitive mutants. Neither isolate synthesized RNA at the nonpermissive temperature (32°), and temperature shift experiments indicated that the restrictive cold-sensitive step in replication occurred during an early viral function. The isolates were unable to complement each other at 32°. Although coinfection between each mutant and parental JHM virus often resulted in decreased yields of both infecting viruses, interference could not be convincingly demonstrated. Both mutants were more thermostable than parental JHM virus and could be distinguished from each other and from parental JHM virus by their thermal inactivation kinetics. The isolates were obtained from single cell clones and therefore presumed to be homogeneous; however, thermal inactivation of one (S-3JHM) indicated the possible existence of a second population. Subclones of virus prepared from each isolate were also found to be thermally stable and to retain the cold sensitive defect. No indication of a second population of S-3JHM virus was detected.
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spelling pubmed-71309922020-04-08 Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion Stohlman, Stephen A. Sakaguchi, Alan Y. Weiner, Leslie P. Virology Article Mouse hepatitis virus, a coronavirus, was rescued from latently infected mouse neuroblastoma cells by polyethylene glycol-induced fusion to permissive cells. The isolated viruses grew to equal or higher titers than parental JHM virus at 39° but were restricted in replication at 32° and hence were cold-sensitive mutants. Neither isolate synthesized RNA at the nonpermissive temperature (32°), and temperature shift experiments indicated that the restrictive cold-sensitive step in replication occurred during an early viral function. The isolates were unable to complement each other at 32°. Although coinfection between each mutant and parental JHM virus often resulted in decreased yields of both infecting viruses, interference could not be convincingly demonstrated. Both mutants were more thermostable than parental JHM virus and could be distinguished from each other and from parental JHM virus by their thermal inactivation kinetics. The isolates were obtained from single cell clones and therefore presumed to be homogeneous; however, thermal inactivation of one (S-3JHM) indicated the possible existence of a second population. Subclones of virus prepared from each isolate were also found to be thermally stable and to retain the cold sensitive defect. No indication of a second population of S-3JHM virus was detected. Published by Elsevier Inc. 1979-10-30 2004-06-09 /pmc/articles/PMC7130992/ /pubmed/228481 http://dx.doi.org/10.1016/0042-6822(79)90567-1 Text en Copyright © 1979 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Stohlman, Stephen A.
Sakaguchi, Alan Y.
Weiner, Leslie P.
Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion
title Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion
title_full Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion
title_fullStr Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion
title_full_unstemmed Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion
title_short Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion
title_sort characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130992/
https://www.ncbi.nlm.nih.gov/pubmed/228481
http://dx.doi.org/10.1016/0042-6822(79)90567-1
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