Mouse hepatitis virus ORF 2a is expressed in the cytosol of infected mouse fibroblasts

A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17CI-1 mouse fibroblasts. The protein is localized prima...

Descripción completa

Detalles Bibliográficos
Autores principales: Zoltick, Philip W., Leibowitz, Julian L., Oleszak, Emilia L., Weiss, Susan R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 1990
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131050/
https://www.ncbi.nlm.nih.gov/pubmed/2154893
http://dx.doi.org/10.1016/0042-6822(90)90114-7
Descripción
Sumario:A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17CI-1 mouse fibroblasts. The protein is localized primarily in the cytosol and not in the membranes. This is consistent with its predicted sequence and potential role as an RNA binding protein.

Ejemplares similares