Cargando…
Characterization of coronavirus JHM variants isolated from wistar furth rats with a viral-induced demyelinating disease
Murine hepatitis virus (MHV) can cause neurological disease when inoculated intracerebrally (ic) into mice and rats. Specifically the JHM strain of MHV (MHV-JHM) generally causes an acute encephalitis when inoculated is into 2-day-old Wistar Furth rats. In contrast, JHM generally produces a chronic...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Inc.
1989
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131095/ https://www.ncbi.nlm.nih.gov/pubmed/2538027 http://dx.doi.org/10.1016/0042-6822(89)90048-2 |
_version_ | 1783517160455274496 |
---|---|
author | Morris, Vincent L. Tieszer, Christina MacKinnon, Joanne Percy, Dean |
author_facet | Morris, Vincent L. Tieszer, Christina MacKinnon, Joanne Percy, Dean |
author_sort | Morris, Vincent L. |
collection | PubMed |
description | Murine hepatitis virus (MHV) can cause neurological disease when inoculated intracerebrally (ic) into mice and rats. Specifically the JHM strain of MHV (MHV-JHM) generally causes an acute encephalitis when inoculated is into 2-day-old Wistar Furth rats. In contrast, JHM generally produces a chronic demyelinating disease with resulting posterior paralysis when inoculated is into 10-day-old Wistar Furth rats. In addition, while JHM readily produces a productive infection in a mouse fibroblast cell line (L-2), it does not form syncytia or replicate well in a tissue cell line of glial origin (G26-24). We have isolated and characterized three MHV-JHM viral variants from the central nervous system of two Wistar Furth rats with a MHV-JHM-induced demyelinating disease. The pattern of viral-specific mRNA for all three of these variants differed from what was observed for the wild-type parental MHV-JHM that had been passaged only in tissue culture. One of these variants, ATIIf cord virus, which induced a chronic demyelinating disease in 2- or 10-day-old intracerebrally inoculated Wistar Furth rats, had a deletion in the coding region of the peplomer glycoprotein mRNA. In addition, this variant formed massive syncytia and replicated well in G26-24 cells. We have not detected this deletion in the other two JHM variants, ATIIf brain virus and ATIIe brain virus. ATIIf brain virus and ATIIe brain virus primarily produced an acute encephalitis when reinoculated into 2-or 10-day-old Wistar Furth rats. In addition, these two variants did not form syncytia and had a reduced ability to replicate in G26-24 cells. |
format | Online Article Text |
id | pubmed-7131095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1989 |
publisher | Published by Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71310952020-04-08 Characterization of coronavirus JHM variants isolated from wistar furth rats with a viral-induced demyelinating disease Morris, Vincent L. Tieszer, Christina MacKinnon, Joanne Percy, Dean Virology Article Murine hepatitis virus (MHV) can cause neurological disease when inoculated intracerebrally (ic) into mice and rats. Specifically the JHM strain of MHV (MHV-JHM) generally causes an acute encephalitis when inoculated is into 2-day-old Wistar Furth rats. In contrast, JHM generally produces a chronic demyelinating disease with resulting posterior paralysis when inoculated is into 10-day-old Wistar Furth rats. In addition, while JHM readily produces a productive infection in a mouse fibroblast cell line (L-2), it does not form syncytia or replicate well in a tissue cell line of glial origin (G26-24). We have isolated and characterized three MHV-JHM viral variants from the central nervous system of two Wistar Furth rats with a MHV-JHM-induced demyelinating disease. The pattern of viral-specific mRNA for all three of these variants differed from what was observed for the wild-type parental MHV-JHM that had been passaged only in tissue culture. One of these variants, ATIIf cord virus, which induced a chronic demyelinating disease in 2- or 10-day-old intracerebrally inoculated Wistar Furth rats, had a deletion in the coding region of the peplomer glycoprotein mRNA. In addition, this variant formed massive syncytia and replicated well in G26-24 cells. We have not detected this deletion in the other two JHM variants, ATIIf brain virus and ATIIe brain virus. ATIIf brain virus and ATIIe brain virus primarily produced an acute encephalitis when reinoculated into 2-or 10-day-old Wistar Furth rats. In addition, these two variants did not form syncytia and had a reduced ability to replicate in G26-24 cells. Published by Elsevier Inc. 1989-03 2004-02-09 /pmc/articles/PMC7131095/ /pubmed/2538027 http://dx.doi.org/10.1016/0042-6822(89)90048-2 Text en Copyright © 1989 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Morris, Vincent L. Tieszer, Christina MacKinnon, Joanne Percy, Dean Characterization of coronavirus JHM variants isolated from wistar furth rats with a viral-induced demyelinating disease |
title | Characterization of coronavirus JHM variants isolated from wistar furth rats with a viral-induced demyelinating disease |
title_full | Characterization of coronavirus JHM variants isolated from wistar furth rats with a viral-induced demyelinating disease |
title_fullStr | Characterization of coronavirus JHM variants isolated from wistar furth rats with a viral-induced demyelinating disease |
title_full_unstemmed | Characterization of coronavirus JHM variants isolated from wistar furth rats with a viral-induced demyelinating disease |
title_short | Characterization of coronavirus JHM variants isolated from wistar furth rats with a viral-induced demyelinating disease |
title_sort | characterization of coronavirus jhm variants isolated from wistar furth rats with a viral-induced demyelinating disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131095/ https://www.ncbi.nlm.nih.gov/pubmed/2538027 http://dx.doi.org/10.1016/0042-6822(89)90048-2 |
work_keys_str_mv | AT morrisvincentl characterizationofcoronavirusjhmvariantsisolatedfromwistarfurthratswithaviralinduceddemyelinatingdisease AT tieszerchristina characterizationofcoronavirusjhmvariantsisolatedfromwistarfurthratswithaviralinduceddemyelinatingdisease AT mackinnonjoanne characterizationofcoronavirusjhmvariantsisolatedfromwistarfurthratswithaviralinduceddemyelinatingdisease AT percydean characterizationofcoronavirusjhmvariantsisolatedfromwistarfurthratswithaviralinduceddemyelinatingdisease |