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Identification of a sorting signal for the regulated secretory pathway at the N-terminus of pro-opiomelanocortin
The N-terminal 26 amino acids of the prohormone pro-opiomelanocortin (POMC) were investigated to determine whether this region has the capacity to act as a sorting signal for the regulated secretory pathway. Constructs were made using the N-terminal 101, 50, 26 or 10 amino acids of POMC fused to the...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Masson SAS
1994
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131109/ https://www.ncbi.nlm.nih.gov/pubmed/7819333 http://dx.doi.org/10.1016/0300-9084(94)90156-2 |
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author | Cool, D.R. Peng Loh, Y. |
author_facet | Cool, D.R. Peng Loh, Y. |
author_sort | Cool, D.R. |
collection | PubMed |
description | The N-terminal 26 amino acids of the prohormone pro-opiomelanocortin (POMC) were investigated to determine whether this region has the capacity to act as a sorting signal for the regulated secretory pathway. Constructs were made using the N-terminal 101, 50, 26 or 10 amino acids of POMC fused to the chloramphenicol acetyltransferase (CAT) reporter protein and expressed in AtT20 cells to show that at least the first 26 amino acids were required to sort CAT to the regulated secretory pathway. Full length POMC was mutated by deleting amino acids 2–26 from the N-terminal region. Analysis of Neuro-2a cells expressing this mutation compared to wild type POMC indicated that these 26 amino acids contain information essential for sorting POMC to the regulated secretory pathway. The results presented here suggest the presence of a conformation-dependent signal in the N-terminal 26 amino acids of POMC responsible for sorting POMC to the regulated secretory pathway. |
format | Online Article Text |
id | pubmed-7131109 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | Published by Elsevier Masson SAS |
record_format | MEDLINE/PubMed |
spelling | pubmed-71311092020-04-08 Identification of a sorting signal for the regulated secretory pathway at the N-terminus of pro-opiomelanocortin Cool, D.R. Peng Loh, Y. Biochimie Article The N-terminal 26 amino acids of the prohormone pro-opiomelanocortin (POMC) were investigated to determine whether this region has the capacity to act as a sorting signal for the regulated secretory pathway. Constructs were made using the N-terminal 101, 50, 26 or 10 amino acids of POMC fused to the chloramphenicol acetyltransferase (CAT) reporter protein and expressed in AtT20 cells to show that at least the first 26 amino acids were required to sort CAT to the regulated secretory pathway. Full length POMC was mutated by deleting amino acids 2–26 from the N-terminal region. Analysis of Neuro-2a cells expressing this mutation compared to wild type POMC indicated that these 26 amino acids contain information essential for sorting POMC to the regulated secretory pathway. The results presented here suggest the presence of a conformation-dependent signal in the N-terminal 26 amino acids of POMC responsible for sorting POMC to the regulated secretory pathway. Published by Elsevier Masson SAS 1994 2003-01-23 /pmc/articles/PMC7131109/ /pubmed/7819333 http://dx.doi.org/10.1016/0300-9084(94)90156-2 Text en Copyright © 1994 Published by Elsevier Masson SAS. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Cool, D.R. Peng Loh, Y. Identification of a sorting signal for the regulated secretory pathway at the N-terminus of pro-opiomelanocortin |
title | Identification of a sorting signal for the regulated secretory pathway at the N-terminus of pro-opiomelanocortin |
title_full | Identification of a sorting signal for the regulated secretory pathway at the N-terminus of pro-opiomelanocortin |
title_fullStr | Identification of a sorting signal for the regulated secretory pathway at the N-terminus of pro-opiomelanocortin |
title_full_unstemmed | Identification of a sorting signal for the regulated secretory pathway at the N-terminus of pro-opiomelanocortin |
title_short | Identification of a sorting signal for the regulated secretory pathway at the N-terminus of pro-opiomelanocortin |
title_sort | identification of a sorting signal for the regulated secretory pathway at the n-terminus of pro-opiomelanocortin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131109/ https://www.ncbi.nlm.nih.gov/pubmed/7819333 http://dx.doi.org/10.1016/0300-9084(94)90156-2 |
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