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Diagnostic Virology Using Electron Microscopic Techniques

This chapter illustrates the development of the use of electron microscopy in viral diagnosis. The field covered is confined to medical viral diagnosis, but parallel developments have taken place in both veterinary and botanical fields and techniques derived from both these sources are also included...

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Autor principal: Field, Anne M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press Inc. Published by Elsevier Inc. 1982
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131171/
https://www.ncbi.nlm.nih.gov/pubmed/6183951
http://dx.doi.org/10.1016/S0065-3527(08)60432-7
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author Field, Anne M.
author_facet Field, Anne M.
author_sort Field, Anne M.
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description This chapter illustrates the development of the use of electron microscopy in viral diagnosis. The field covered is confined to medical viral diagnosis, but parallel developments have taken place in both veterinary and botanical fields and techniques derived from both these sources are also included where relevant. It is reported that the scanning transmission mode of operation, which can induce image contrast changes electronically, may enhance studies with unstained sections and perhaps facilitate thin section immune electron microscopy (IEM). The application of negative stain IEM has been particularly useful for the study of the antigenic nature of some of the newly discovered noncultivable viruses. Viral antigens can also be detected in thin sections of infected cells by IEM with suitably labeled specific antibodies. Confirmation of viral infection by electron microscopy on tissues originally processed for light microscopy is also frequently useful.
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spelling pubmed-71311712020-04-08 Diagnostic Virology Using Electron Microscopic Techniques Field, Anne M. Adv Virus Res Article This chapter illustrates the development of the use of electron microscopy in viral diagnosis. The field covered is confined to medical viral diagnosis, but parallel developments have taken place in both veterinary and botanical fields and techniques derived from both these sources are also included where relevant. It is reported that the scanning transmission mode of operation, which can induce image contrast changes electronically, may enhance studies with unstained sections and perhaps facilitate thin section immune electron microscopy (IEM). The application of negative stain IEM has been particularly useful for the study of the antigenic nature of some of the newly discovered noncultivable viruses. Viral antigens can also be detected in thin sections of infected cells by IEM with suitably labeled specific antibodies. Confirmation of viral infection by electron microscopy on tissues originally processed for light microscopy is also frequently useful. Academic Press Inc. Published by Elsevier Inc. 1982 2008-04-09 /pmc/articles/PMC7131171/ /pubmed/6183951 http://dx.doi.org/10.1016/S0065-3527(08)60432-7 Text en © 1982 Academic Press Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Field, Anne M.
Diagnostic Virology Using Electron Microscopic Techniques
title Diagnostic Virology Using Electron Microscopic Techniques
title_full Diagnostic Virology Using Electron Microscopic Techniques
title_fullStr Diagnostic Virology Using Electron Microscopic Techniques
title_full_unstemmed Diagnostic Virology Using Electron Microscopic Techniques
title_short Diagnostic Virology Using Electron Microscopic Techniques
title_sort diagnostic virology using electron microscopic techniques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131171/
https://www.ncbi.nlm.nih.gov/pubmed/6183951
http://dx.doi.org/10.1016/S0065-3527(08)60432-7
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