Studies on canine parvovirus infection: preparation of challenge virus

Two techniques, adsorption on to hydroxylapatite and density gradient centrifugation, were investigated as prospective methods for the large scale purification of canine parvovirus from faecal suspensions. Adsorption with hydroxylapatite successfully removed virus from faecal material. However, the...

Descripción completa

Detalles Bibliográficos
Autores principales: MACARTNEY, L., McCANDLISH, I.A.P., THOMPSON, H., CORNWELL, H.J.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd. 1988
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131241/
https://www.ncbi.nlm.nih.gov/pubmed/2848300
http://dx.doi.org/10.1016/S0034-5288(18)30926-3
_version_ 1783517193990832128
author MACARTNEY, L.
McCANDLISH, I.A.P.
THOMPSON, H.
CORNWELL, H.J.C.
author_facet MACARTNEY, L.
McCANDLISH, I.A.P.
THOMPSON, H.
CORNWELL, H.J.C.
author_sort MACARTNEY, L.
collection PubMed
description Two techniques, adsorption on to hydroxylapatite and density gradient centrifugation, were investigated as prospective methods for the large scale purification of canine parvovirus from faecal suspensions. Adsorption with hydroxylapatite successfully removed virus from faecal material. However, the resultant virus was contaminated and some virus was left behind in the faecal suspension. Repeated adsorption with hydroxylapatite appeared to result in some damage to the virus particles. In contrast, density gradient centrifugation provided a simple, economical method of purification which yielded uncontaminated, infectious virus. The final method, using both isopyknic and rate zonal centrifugation is described.
format Online
Article
Text
id pubmed-7131241
institution National Center for Biotechnology Information
language English
publishDate 1988
publisher Published by Elsevier Ltd.
record_format MEDLINE/PubMed
spelling pubmed-71312412020-04-08 Studies on canine parvovirus infection: preparation of challenge virus MACARTNEY, L. McCANDLISH, I.A.P. THOMPSON, H. CORNWELL, H.J.C. Res Vet Sci Article Two techniques, adsorption on to hydroxylapatite and density gradient centrifugation, were investigated as prospective methods for the large scale purification of canine parvovirus from faecal suspensions. Adsorption with hydroxylapatite successfully removed virus from faecal material. However, the resultant virus was contaminated and some virus was left behind in the faecal suspension. Repeated adsorption with hydroxylapatite appeared to result in some damage to the virus particles. In contrast, density gradient centrifugation provided a simple, economical method of purification which yielded uncontaminated, infectious virus. The final method, using both isopyknic and rate zonal centrifugation is described. Published by Elsevier Ltd. 1988-09 2018-08-23 /pmc/articles/PMC7131241/ /pubmed/2848300 http://dx.doi.org/10.1016/S0034-5288(18)30926-3 Text en Copyright © 1988 Published by Elsevier Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
MACARTNEY, L.
McCANDLISH, I.A.P.
THOMPSON, H.
CORNWELL, H.J.C.
Studies on canine parvovirus infection: preparation of challenge virus
title Studies on canine parvovirus infection: preparation of challenge virus
title_full Studies on canine parvovirus infection: preparation of challenge virus
title_fullStr Studies on canine parvovirus infection: preparation of challenge virus
title_full_unstemmed Studies on canine parvovirus infection: preparation of challenge virus
title_short Studies on canine parvovirus infection: preparation of challenge virus
title_sort studies on canine parvovirus infection: preparation of challenge virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131241/
https://www.ncbi.nlm.nih.gov/pubmed/2848300
http://dx.doi.org/10.1016/S0034-5288(18)30926-3
work_keys_str_mv AT macartneyl studiesoncanineparvovirusinfectionpreparationofchallengevirus
AT mccandlishiap studiesoncanineparvovirusinfectionpreparationofchallengevirus
AT thompsonh studiesoncanineparvovirusinfectionpreparationofchallengevirus
AT cornwellhjc studiesoncanineparvovirusinfectionpreparationofchallengevirus

Ejemplares similares