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Intracellular murine hepatitis virus-specific RNAs contain common sequences
A major polyadenylated viral RNA of approximately 0.8 × 10(6) daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-fre...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Inc.
1981
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131256/ https://www.ncbi.nlm.nih.gov/pubmed/6114592 http://dx.doi.org/10.1016/0042-6822(81)90305-6 |
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author | Cheley, Steve Anderson, Robert Cupples, Margaret J. Lee Chan, Edwin C.M. Morris, Vincent L. |
author_facet | Cheley, Steve Anderson, Robert Cupples, Margaret J. Lee Chan, Edwin C.M. Morris, Vincent L. |
author_sort | Cheley, Steve |
collection | PubMed |
description | A major polyadenylated viral RNA of approximately 0.8 × 10(6) daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded (32)P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 10(6) daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences. |
format | Online Article Text |
id | pubmed-7131256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1981 |
publisher | Published by Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71312562020-04-08 Intracellular murine hepatitis virus-specific RNAs contain common sequences Cheley, Steve Anderson, Robert Cupples, Margaret J. Lee Chan, Edwin C.M. Morris, Vincent L. Virology Article A major polyadenylated viral RNA of approximately 0.8 × 10(6) daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded (32)P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 10(6) daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences. Published by Elsevier Inc. 1981-07-30 2004-06-09 /pmc/articles/PMC7131256/ /pubmed/6114592 http://dx.doi.org/10.1016/0042-6822(81)90305-6 Text en Copyright © 1981 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Cheley, Steve Anderson, Robert Cupples, Margaret J. Lee Chan, Edwin C.M. Morris, Vincent L. Intracellular murine hepatitis virus-specific RNAs contain common sequences |
title | Intracellular murine hepatitis virus-specific RNAs contain common sequences |
title_full | Intracellular murine hepatitis virus-specific RNAs contain common sequences |
title_fullStr | Intracellular murine hepatitis virus-specific RNAs contain common sequences |
title_full_unstemmed | Intracellular murine hepatitis virus-specific RNAs contain common sequences |
title_short | Intracellular murine hepatitis virus-specific RNAs contain common sequences |
title_sort | intracellular murine hepatitis virus-specific rnas contain common sequences |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131256/ https://www.ncbi.nlm.nih.gov/pubmed/6114592 http://dx.doi.org/10.1016/0042-6822(81)90305-6 |
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