Comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein

We have examined six different JHMV variants, sp-4 (recloned wt JHMV), cl-2, CNSV, DL, DS, and JHM-X, in terms of the sizes of the mRNA3 and E2 glycoprotein as well as their reactivity to a panel of monoclonal antibodies to the E2 glycoprotein. Two of these variants, sp-4 and JHM-X, were found to ha...

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Detalles Bibliográficos
Autores principales: Taguchi, Fumihiro, Flemingt, John O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 1989
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131304/
https://www.ncbi.nlm.nih.gov/pubmed/2538034
http://dx.doi.org/10.1016/0042-6822(89)90061-5
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author Taguchi, Fumihiro
Flemingt, John O.
author_facet Taguchi, Fumihiro
Flemingt, John O.
author_sort Taguchi, Fumihiro
collection PubMed
description We have examined six different JHMV variants, sp-4 (recloned wt JHMV), cl-2, CNSV, DL, DS, and JHM-X, in terms of the sizes of the mRNA3 and E2 glycoprotein as well as their reactivity to a panel of monoclonal antibodies to the E2 glycoprotein. Two of these variants, sp-4 and JHM-X, were found to have smaller mRNA3 and E2 glycoprotein species compared with those of the other four variants. In addition, sp-4 and JHM-X were distinguished from the other four variants by their inability to bind to monoclonal antibodies recognizing two antigenic domains of the E2 molecule. Thus, six JHMV variants could clearly be divided into two groups with respect to the size and antigenicity of their E2 glycoproteins.
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spelling pubmed-71313042020-04-08 Comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein Taguchi, Fumihiro Flemingt, John O. Virology Article We have examined six different JHMV variants, sp-4 (recloned wt JHMV), cl-2, CNSV, DL, DS, and JHM-X, in terms of the sizes of the mRNA3 and E2 glycoprotein as well as their reactivity to a panel of monoclonal antibodies to the E2 glycoprotein. Two of these variants, sp-4 and JHM-X, were found to have smaller mRNA3 and E2 glycoprotein species compared with those of the other four variants. In addition, sp-4 and JHM-X were distinguished from the other four variants by their inability to bind to monoclonal antibodies recognizing two antigenic domains of the E2 molecule. Thus, six JHMV variants could clearly be divided into two groups with respect to the size and antigenicity of their E2 glycoproteins. Published by Elsevier Inc. 1989-03 2004-02-09 /pmc/articles/PMC7131304/ /pubmed/2538034 http://dx.doi.org/10.1016/0042-6822(89)90061-5 Text en Copyright © 1989 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Taguchi, Fumihiro
Flemingt, John O.
Comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein
title Comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein
title_full Comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein
title_fullStr Comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein
title_full_unstemmed Comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein
title_short Comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein
title_sort comparison of six different murine coronavirus jhm variants by monoclonal antibodies against the e2 glycoprotein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131304/
https://www.ncbi.nlm.nih.gov/pubmed/2538034
http://dx.doi.org/10.1016/0042-6822(89)90061-5
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