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PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine
Porcine deltacoronavirus (PDCoV) was first identified in Hong Kong in a regional surveillance study for Coronaviruses in 2012 and was detected for the first time in United States (US) swine in February 2014. However, it remains unknown if PDCoV had been introduced into the US prior to that time peri...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131395/ https://www.ncbi.nlm.nih.gov/pubmed/26119039 http://dx.doi.org/10.1016/j.vetmic.2015.06.005 |
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author | Sinha, Avanti Gauger, Phillip Zhang, Jianqiang Yoon, Kyoung-Jin Harmon, Karen |
author_facet | Sinha, Avanti Gauger, Phillip Zhang, Jianqiang Yoon, Kyoung-Jin Harmon, Karen |
author_sort | Sinha, Avanti |
collection | PubMed |
description | Porcine deltacoronavirus (PDCoV) was first identified in Hong Kong in a regional surveillance study for Coronaviruses in 2012 and was detected for the first time in United States (US) swine in February 2014. However, it remains unknown if PDCoV had been introduced into the US prior to that time period. In the present study, 1734 clinical samples (903 cases) submitted to the Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) for enteric disease diagnosis between October 2012 and December 2013 were tested retrospectively for PDCoV using a virus-specific real-time reverse transcription (RT) PCR targeting conserved region of the membrane gene. PDCoV genome was first detected in a fecal sample collected on August 19th 2013 from Minnesota. Subsequently, PDCoV was observed in samples collected on August 20th and August 27th from Iowa and on August 29th from Illinois. Therefore, with available samples submitted to the ISU VDL, it can be inferred that PDCoV has been present in US swine at least since August 2013. |
format | Online Article Text |
id | pubmed-7131395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71313952020-04-08 PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine Sinha, Avanti Gauger, Phillip Zhang, Jianqiang Yoon, Kyoung-Jin Harmon, Karen Vet Microbiol Article Porcine deltacoronavirus (PDCoV) was first identified in Hong Kong in a regional surveillance study for Coronaviruses in 2012 and was detected for the first time in United States (US) swine in February 2014. However, it remains unknown if PDCoV had been introduced into the US prior to that time period. In the present study, 1734 clinical samples (903 cases) submitted to the Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) for enteric disease diagnosis between October 2012 and December 2013 were tested retrospectively for PDCoV using a virus-specific real-time reverse transcription (RT) PCR targeting conserved region of the membrane gene. PDCoV genome was first detected in a fecal sample collected on August 19th 2013 from Minnesota. Subsequently, PDCoV was observed in samples collected on August 20th and August 27th from Iowa and on August 29th from Illinois. Therefore, with available samples submitted to the ISU VDL, it can be inferred that PDCoV has been present in US swine at least since August 2013. Elsevier B.V. 2015-09-30 2015-06-10 /pmc/articles/PMC7131395/ /pubmed/26119039 http://dx.doi.org/10.1016/j.vetmic.2015.06.005 Text en Copyright © 2015 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Sinha, Avanti Gauger, Phillip Zhang, Jianqiang Yoon, Kyoung-Jin Harmon, Karen PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine |
title | PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine |
title_full | PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine |
title_fullStr | PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine |
title_full_unstemmed | PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine |
title_short | PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine |
title_sort | pcr-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in us swine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131395/ https://www.ncbi.nlm.nih.gov/pubmed/26119039 http://dx.doi.org/10.1016/j.vetmic.2015.06.005 |
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