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Parent-collected respiratory specimens—A novel method for respiratory virus and vaccine efficacy research

Population-based respiratory research and vaccine efficacy studies have previously required clinic or home visits when a subject had an acute respiratory illness. This method may mean parents are unwilling to enrol their child or report an illness of interest. We conducted a community-based cohort s...

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Detalles Bibliográficos
Autores principales: Lambert, Stephen B., Allen, Kelly M., Nolan, Terence M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131404/
https://www.ncbi.nlm.nih.gov/pubmed/18329758
http://dx.doi.org/10.1016/j.vaccine.2008.01.055
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author Lambert, Stephen B.
Allen, Kelly M.
Nolan, Terence M.
author_facet Lambert, Stephen B.
Allen, Kelly M.
Nolan, Terence M.
author_sort Lambert, Stephen B.
collection PubMed
description Population-based respiratory research and vaccine efficacy studies have previously required clinic or home visits when a subject had an acute respiratory illness. This method may mean parents are unwilling to enrol their child or report an illness of interest. We conducted a community-based cohort study into respiratory illnesses in 234 pre-school aged children using parent-collected specimens. Between January 2003 and January 2004 there were 563 specimens collected from 730 identified illnesses and these were tested using a panel of respiratory virus polymerase chain reaction (PCR) assays; 409 (73%) were positive for any virus. Specimens were not more likely to be positive when collected by a healthcare worker parent, when they included a throat swab, or when a very good collection technique was reported. A delay from illness onset to specimen collection of up to 5 days did not appear to impact on sensitivity of virus identification, but a delay of six or more days with minor delays in testing saw positivity fall. Combined with daily symptom diary completion and PCR testing, parent-collected specimens are an efficient and acceptable method for the conduct of future vaccine efficacy studies and other community-based respiratory virus research.
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spelling pubmed-71314042020-04-08 Parent-collected respiratory specimens—A novel method for respiratory virus and vaccine efficacy research Lambert, Stephen B. Allen, Kelly M. Nolan, Terence M. Vaccine Article Population-based respiratory research and vaccine efficacy studies have previously required clinic or home visits when a subject had an acute respiratory illness. This method may mean parents are unwilling to enrol their child or report an illness of interest. We conducted a community-based cohort study into respiratory illnesses in 234 pre-school aged children using parent-collected specimens. Between January 2003 and January 2004 there were 563 specimens collected from 730 identified illnesses and these were tested using a panel of respiratory virus polymerase chain reaction (PCR) assays; 409 (73%) were positive for any virus. Specimens were not more likely to be positive when collected by a healthcare worker parent, when they included a throat swab, or when a very good collection technique was reported. A delay from illness onset to specimen collection of up to 5 days did not appear to impact on sensitivity of virus identification, but a delay of six or more days with minor delays in testing saw positivity fall. Combined with daily symptom diary completion and PCR testing, parent-collected specimens are an efficient and acceptable method for the conduct of future vaccine efficacy studies and other community-based respiratory virus research. Elsevier Ltd. 2008-03-28 2008-02-22 /pmc/articles/PMC7131404/ /pubmed/18329758 http://dx.doi.org/10.1016/j.vaccine.2008.01.055 Text en Copyright © 2008 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Lambert, Stephen B.
Allen, Kelly M.
Nolan, Terence M.
Parent-collected respiratory specimens—A novel method for respiratory virus and vaccine efficacy research
title Parent-collected respiratory specimens—A novel method for respiratory virus and vaccine efficacy research
title_full Parent-collected respiratory specimens—A novel method for respiratory virus and vaccine efficacy research
title_fullStr Parent-collected respiratory specimens—A novel method for respiratory virus and vaccine efficacy research
title_full_unstemmed Parent-collected respiratory specimens—A novel method for respiratory virus and vaccine efficacy research
title_short Parent-collected respiratory specimens—A novel method for respiratory virus and vaccine efficacy research
title_sort parent-collected respiratory specimens—a novel method for respiratory virus and vaccine efficacy research
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131404/
https://www.ncbi.nlm.nih.gov/pubmed/18329758
http://dx.doi.org/10.1016/j.vaccine.2008.01.055
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