Chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (HIG) test for viral antibodies

The sensitivity of the hemolysis-in-gel (HIG) test with rubella antigen is not improved by chemical linkage of the virus to the erythrocytes, and after such modification, IgM specific antibodies are not detectable. In the influenza HIG test with tetraazotized o-dianisidine (TOD), chromic chloride an...

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Detalles Bibliográficos
Autores principales: Steinmann, J., Marzock, H.-J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1983
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131419/
https://www.ncbi.nlm.nih.gov/pubmed/6302167
http://dx.doi.org/10.1016/0022-1759(83)90034-0
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author Steinmann, J.
Marzock, H.-J.
author_facet Steinmann, J.
Marzock, H.-J.
author_sort Steinmann, J.
collection PubMed
description The sensitivity of the hemolysis-in-gel (HIG) test with rubella antigen is not improved by chemical linkage of the virus to the erythrocytes, and after such modification, IgM specific antibodies are not detectable. In the influenza HIG test with tetraazotized o-dianisidine (TOD), chromic chloride and potassium periodate as coupling reagents, increased sensitivity was observed with allantoic fluid of infected eggs as antigen. If Tween-ether treated hemagglutinin is used in the HIG test, zones of hemolysis are detectable only after treatment of the erythrocytes with TOD, chromic chloride and potassium periodate.
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spelling pubmed-71314192020-04-08 Chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (HIG) test for viral antibodies Steinmann, J. Marzock, H.-J. J Immunol Methods Article The sensitivity of the hemolysis-in-gel (HIG) test with rubella antigen is not improved by chemical linkage of the virus to the erythrocytes, and after such modification, IgM specific antibodies are not detectable. In the influenza HIG test with tetraazotized o-dianisidine (TOD), chromic chloride and potassium periodate as coupling reagents, increased sensitivity was observed with allantoic fluid of infected eggs as antigen. If Tween-ether treated hemagglutinin is used in the HIG test, zones of hemolysis are detectable only after treatment of the erythrocytes with TOD, chromic chloride and potassium periodate. Published by Elsevier B.V. 1983 2002-11-12 /pmc/articles/PMC7131419/ /pubmed/6302167 http://dx.doi.org/10.1016/0022-1759(83)90034-0 Text en Copyright © 1983 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Steinmann, J.
Marzock, H.-J.
Chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (HIG) test for viral antibodies
title Chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (HIG) test for viral antibodies
title_full Chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (HIG) test for viral antibodies
title_fullStr Chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (HIG) test for viral antibodies
title_full_unstemmed Chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (HIG) test for viral antibodies
title_short Chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (HIG) test for viral antibodies
title_sort chemical linkage of erythrocytes and viral antigen in the hemolysis-in-gel (hig) test for viral antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131419/
https://www.ncbi.nlm.nih.gov/pubmed/6302167
http://dx.doi.org/10.1016/0022-1759(83)90034-0
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AT marzockhj chemicallinkageoferythrocytesandviralantigeninthehemolysisingelhigtestforviralantibodies

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