Détection des rotavirus humains dans les selles : Comparaison de cinq techniques

The authors have tested 118 stools by five techniques used for the detection of rotaviruses in human faeces : electron microscopy, latex agglutination (Slidex Rota-kit : BioMérieux ; Rotalex : Orion Diagnostica) and ELISA (Enzygnost : Behring ; Bio-Enza-Bead : Bionetics). Reproducibility and specifi...

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Detalles Bibliográficos
Autores principales: Baillargeau, E., Ranger, S., Agius, G., Samb, A, Castets, M., Rekas, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Masson SAS 1985
Materias:
Travaux Originaux
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131474/
http://dx.doi.org/10.1016/S0399-077X(85)80323-1
Descripción
Sumario:The authors have tested 118 stools by five techniques used for the detection of rotaviruses in human faeces : electron microscopy, latex agglutination (Slidex Rota-kit : BioMérieux ; Rotalex : Orion Diagnostica) and ELISA (Enzygnost : Behring ; Bio-Enza-Bead : Bionetics). Reproducibility and specificity for all techniques were satisfactory. Comparison of the methods showed Enzygnost to be the most sensitive (89 %), followed by the two agglutination tests, then electron microscopy and least sensitive the Bio-Enza-Bead (56 %). For large — scale screening of faeces, it appeared that the latex agglutination test were preferable for reasons of simplicity, speed and cost.

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