Détection des rotavirus humains dans les selles : Comparaison de cinq techniques

The authors have tested 118 stools by five techniques used for the detection of rotaviruses in human faeces : electron microscopy, latex agglutination (Slidex Rota-kit : BioMérieux ; Rotalex : Orion Diagnostica) and ELISA (Enzygnost : Behring ; Bio-Enza-Bead : Bionetics). Reproducibility and specifi...

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Detalles Bibliográficos
Autores principales: Baillargeau, E., Ranger, S., Agius, G., Samb, A, Castets, M., Rekas, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Masson SAS 1985
Materias:
Travaux Originaux
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131474/
http://dx.doi.org/10.1016/S0399-077X(85)80323-1
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author Baillargeau, E.
Ranger, S.
Agius, G.
Samb, A
Castets, M.
Rekas, B.
author_facet Baillargeau, E.
Ranger, S.
Agius, G.
Samb, A
Castets, M.
Rekas, B.
author_sort Baillargeau, E.
collection PubMed
description The authors have tested 118 stools by five techniques used for the detection of rotaviruses in human faeces : electron microscopy, latex agglutination (Slidex Rota-kit : BioMérieux ; Rotalex : Orion Diagnostica) and ELISA (Enzygnost : Behring ; Bio-Enza-Bead : Bionetics). Reproducibility and specificity for all techniques were satisfactory. Comparison of the methods showed Enzygnost to be the most sensitive (89 %), followed by the two agglutination tests, then electron microscopy and least sensitive the Bio-Enza-Bead (56 %). For large — scale screening of faeces, it appeared that the latex agglutination test were preferable for reasons of simplicity, speed and cost.
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spelling pubmed-71314742020-04-08 Détection des rotavirus humains dans les selles : Comparaison de cinq techniques Baillargeau, E. Ranger, S. Agius, G. Samb, A Castets, M. Rekas, B. Med Mal Infect Travaux Originaux The authors have tested 118 stools by five techniques used for the detection of rotaviruses in human faeces : electron microscopy, latex agglutination (Slidex Rota-kit : BioMérieux ; Rotalex : Orion Diagnostica) and ELISA (Enzygnost : Behring ; Bio-Enza-Bead : Bionetics). Reproducibility and specificity for all techniques were satisfactory. Comparison of the methods showed Enzygnost to be the most sensitive (89 %), followed by the two agglutination tests, then electron microscopy and least sensitive the Bio-Enza-Bead (56 %). For large — scale screening of faeces, it appeared that the latex agglutination test were preferable for reasons of simplicity, speed and cost. Published by Elsevier Masson SAS 1985-12 2005-06-20 /pmc/articles/PMC7131474/ http://dx.doi.org/10.1016/S0399-077X(85)80323-1 Text en Copyright © 1985 Published by Elsevier Masson SAS. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Travaux Originaux
Baillargeau, E.
Ranger, S.
Agius, G.
Samb, A
Castets, M.
Rekas, B.
Détection des rotavirus humains dans les selles : Comparaison de cinq techniques
title Détection des rotavirus humains dans les selles : Comparaison de cinq techniques
title_full Détection des rotavirus humains dans les selles : Comparaison de cinq techniques
title_fullStr Détection des rotavirus humains dans les selles : Comparaison de cinq techniques
title_full_unstemmed Détection des rotavirus humains dans les selles : Comparaison de cinq techniques
title_short Détection des rotavirus humains dans les selles : Comparaison de cinq techniques
title_sort détection des rotavirus humains dans les selles : comparaison de cinq techniques
topic Travaux Originaux
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131474/
http://dx.doi.org/10.1016/S0399-077X(85)80323-1
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AT samba detectiondesrotavirushumainsdanslessellescomparaisondecinqtechniques
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