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Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus

Monoclonal antibodies were produced to JHMV-DL, a neurotropic member of the mouse hepatitis virus (MHV) or murine coronavirus group. Of 23 antibodies isolated, 10 were specific for the major envelope glycoprotein, gp180/90, 10 for the nucleocapsid protein, pp60, and 3 for the minor envelope glycopro...

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Autores principales: Fleming, John O., Stohlman, Stephen A., Harmon, Richard C., McLai, Michael, Frelinger, Jeffrey A., Weiner, Leslie P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 1983
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131482/
https://www.ncbi.nlm.nih.gov/pubmed/6318433
http://dx.doi.org/10.1016/0042-6822(83)90498-1
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author Fleming, John O.
Stohlman, Stephen A.
Harmon, Richard C.
McLai, Michael
Frelinger, Jeffrey A.
Weiner, Leslie P.
author_facet Fleming, John O.
Stohlman, Stephen A.
Harmon, Richard C.
McLai, Michael
Frelinger, Jeffrey A.
Weiner, Leslie P.
author_sort Fleming, John O.
collection PubMed
description Monoclonal antibodies were produced to JHMV-DL, a neurotropic member of the mouse hepatitis virus (MHV) or murine coronavirus group. Of 23 antibodies isolated, 10 were specific for the major envelope glycoprotein, gp180/90, 10 for the nucleocapsid protein, pp60, and 3 for the minor envelope glycoprotein, gp25. Eleven different MHV isolates were used in antibody binding assays to study antigenic relationships among the viruses. Each MHV isolate tested had a unique pattern of antibody binding, indicating that each is a distinct strain. Conservation of JHMV-DL antigenic determinants varied among the three proteins, with pp60 showing intermediate conservation, gp180/90 little conservation, and gp25 marked conservation in the different MHV strains. Monoclonal antibodies to pp60 proved most useful in delineating antigenic relationships among MHV strains. These antigenic groups correlated with pathogenic types, indicating that pp60 may be one of the gene products which mediates the distinct disease patterns manifested by different murine coronaviruses.
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spelling pubmed-71314822020-04-08 Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus Fleming, John O. Stohlman, Stephen A. Harmon, Richard C. McLai, Michael Frelinger, Jeffrey A. Weiner, Leslie P. Virology Article Monoclonal antibodies were produced to JHMV-DL, a neurotropic member of the mouse hepatitis virus (MHV) or murine coronavirus group. Of 23 antibodies isolated, 10 were specific for the major envelope glycoprotein, gp180/90, 10 for the nucleocapsid protein, pp60, and 3 for the minor envelope glycoprotein, gp25. Eleven different MHV isolates were used in antibody binding assays to study antigenic relationships among the viruses. Each MHV isolate tested had a unique pattern of antibody binding, indicating that each is a distinct strain. Conservation of JHMV-DL antigenic determinants varied among the three proteins, with pp60 showing intermediate conservation, gp180/90 little conservation, and gp25 marked conservation in the different MHV strains. Monoclonal antibodies to pp60 proved most useful in delineating antigenic relationships among MHV strains. These antigenic groups correlated with pathogenic types, indicating that pp60 may be one of the gene products which mediates the distinct disease patterns manifested by different murine coronaviruses. Published by Elsevier Inc. 1983-12 2004-06-09 /pmc/articles/PMC7131482/ /pubmed/6318433 http://dx.doi.org/10.1016/0042-6822(83)90498-1 Text en Copyright © 1983 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Fleming, John O.
Stohlman, Stephen A.
Harmon, Richard C.
McLai, Michael
Frelinger, Jeffrey A.
Weiner, Leslie P.
Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus
title Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus
title_full Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus
title_fullStr Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus
title_full_unstemmed Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus
title_short Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus
title_sort antigenic relationships of murine coronaviruses: analysis using monoclonal antibodies to jhm (mhv-4) virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131482/
https://www.ncbi.nlm.nih.gov/pubmed/6318433
http://dx.doi.org/10.1016/0042-6822(83)90498-1
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