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A Murine Coronavirus MHV-S Isolate from Persistently infected Cells Has a Leader and Two Consensus Sequences between the M and N Genes
A plaque-cloned mouse hepatitis virus mutant, MHV-S No. 8, was isolated from Ki-BALB cells persistently infected with MHV-S. The mRNAs 1 to 6 were larger in the mutant, whereas there was no difference between the two viruses in the size of the smallest mRNA, mRNA 7. Sequence analyses of the genomic...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Academic Press.
1994
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131508/ https://www.ncbi.nlm.nih.gov/pubmed/8259671 http://dx.doi.org/10.1006/viro.1994.1041 |
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author | Taguchi, Fumihiro Ikeda, Toshio Makino, Shinji Yoshikura, Hiroshi |
author_facet | Taguchi, Fumihiro Ikeda, Toshio Makino, Shinji Yoshikura, Hiroshi |
author_sort | Taguchi, Fumihiro |
collection | PubMed |
description | A plaque-cloned mouse hepatitis virus mutant, MHV-S No. 8, was isolated from Ki-BALB cells persistently infected with MHV-S. The mRNAs 1 to 6 were larger in the mutant, whereas there was no difference between the two viruses in the size of the smallest mRNA, mRNA 7. Sequence analyses of the genomic RNA, mRNA 6, and mRNA 7 of the two viruses revealed that an additional 111 nt were inserted just upstream of the intergenic consensus sequence preceding the N gene in MHV-S No. 8. The inserted region consisted of two different parts; the 3′-most 30 nt corresponded to nucleotides 28 to 57 of the leader sequence and the 5′-most 81 nt corresponded to nucleotides 58 to 138 of mRNA 7. This structure of No. 8 was most likely generated by RNA-RNA recombination between genomic RNA and subgenomic RNA species. The nucleotide insertion in the intergenic sequence between genes M and N resulted in two consensus sequences separated by 111 nt. Primer extension analysis revealed that the amount of a slightly larger, subgenomic mRNA resulting from initiation of synthesis at the upstream consensus sequence was only 5% of the usual sized mRNA 7 initiated from the downstream consensus sequence. |
format | Online Article Text |
id | pubmed-7131508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | Academic Press. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71315082020-04-08 A Murine Coronavirus MHV-S Isolate from Persistently infected Cells Has a Leader and Two Consensus Sequences between the M and N Genes Taguchi, Fumihiro Ikeda, Toshio Makino, Shinji Yoshikura, Hiroshi Virology Short Communications A plaque-cloned mouse hepatitis virus mutant, MHV-S No. 8, was isolated from Ki-BALB cells persistently infected with MHV-S. The mRNAs 1 to 6 were larger in the mutant, whereas there was no difference between the two viruses in the size of the smallest mRNA, mRNA 7. Sequence analyses of the genomic RNA, mRNA 6, and mRNA 7 of the two viruses revealed that an additional 111 nt were inserted just upstream of the intergenic consensus sequence preceding the N gene in MHV-S No. 8. The inserted region consisted of two different parts; the 3′-most 30 nt corresponded to nucleotides 28 to 57 of the leader sequence and the 5′-most 81 nt corresponded to nucleotides 58 to 138 of mRNA 7. This structure of No. 8 was most likely generated by RNA-RNA recombination between genomic RNA and subgenomic RNA species. The nucleotide insertion in the intergenic sequence between genes M and N resulted in two consensus sequences separated by 111 nt. Primer extension analysis revealed that the amount of a slightly larger, subgenomic mRNA resulting from initiation of synthesis at the upstream consensus sequence was only 5% of the usual sized mRNA 7 initiated from the downstream consensus sequence. Academic Press. 1994-01 2002-05-25 /pmc/articles/PMC7131508/ /pubmed/8259671 http://dx.doi.org/10.1006/viro.1994.1041 Text en Copyright © 1994 Academic Press. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Short Communications Taguchi, Fumihiro Ikeda, Toshio Makino, Shinji Yoshikura, Hiroshi A Murine Coronavirus MHV-S Isolate from Persistently infected Cells Has a Leader and Two Consensus Sequences between the M and N Genes |
title | A Murine Coronavirus MHV-S Isolate from Persistently infected Cells Has a Leader and Two Consensus Sequences between the M and N Genes |
title_full | A Murine Coronavirus MHV-S Isolate from Persistently infected Cells Has a Leader and Two Consensus Sequences between the M and N Genes |
title_fullStr | A Murine Coronavirus MHV-S Isolate from Persistently infected Cells Has a Leader and Two Consensus Sequences between the M and N Genes |
title_full_unstemmed | A Murine Coronavirus MHV-S Isolate from Persistently infected Cells Has a Leader and Two Consensus Sequences between the M and N Genes |
title_short | A Murine Coronavirus MHV-S Isolate from Persistently infected Cells Has a Leader and Two Consensus Sequences between the M and N Genes |
title_sort | murine coronavirus mhv-s isolate from persistently infected cells has a leader and two consensus sequences between the m and n genes |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131508/ https://www.ncbi.nlm.nih.gov/pubmed/8259671 http://dx.doi.org/10.1006/viro.1994.1041 |
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