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cis acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus

The pol gene of the Moloney murine leukemia virus (M-MuLV) is expressed as a Gag-Pol fusion protein through an in-frame suppression of the UAG termination codon located between the two genes. The role of nucleotide context in suppression was investigated, in a rabbit reticulocyte lysate translation...

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Detalles Bibliográficos
Autores principales: Honigman, Alik, Wolf, Dana, Yaish, Shoshanit, Falk, Haya, Panet, Amos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 1991
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131665/
https://www.ncbi.nlm.nih.gov/pubmed/2053284
http://dx.doi.org/10.1016/0042-6822(91)90144-Z
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author Honigman, Alik
Wolf, Dana
Yaish, Shoshanit
Falk, Haya
Panet, Amos
author_facet Honigman, Alik
Wolf, Dana
Yaish, Shoshanit
Falk, Haya
Panet, Amos
author_sort Honigman, Alik
collection PubMed
description The pol gene of the Moloney murine leukemia virus (M-MuLV) is expressed as a Gag-Pol fusion protein through an in-frame suppression of the UAG termination codon located between the two genes. The role of nucleotide context in suppression was investigated, in a rabbit reticulocyte lysate translation system, using site-directed mutagenesis. The results indicate that the translational readthrough is mediated by at least 50 bases long RNA sequence located 3′ to the gag UAG termination codon. Within this sequence a short purine-rich sequence adjacent to the amber codon, highly conserved among different retroviruses, appears essential for M-MuLV suppression. Two alternative putative stem and loop like RNA structures can be drawn at the gag-pol junction, one abutting the gag UAG codon, and the second downstream to it. None of these structures appears to be important to the suppression process.
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spelling pubmed-71316652020-04-08 cis acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus Honigman, Alik Wolf, Dana Yaish, Shoshanit Falk, Haya Panet, Amos Virology Article The pol gene of the Moloney murine leukemia virus (M-MuLV) is expressed as a Gag-Pol fusion protein through an in-frame suppression of the UAG termination codon located between the two genes. The role of nucleotide context in suppression was investigated, in a rabbit reticulocyte lysate translation system, using site-directed mutagenesis. The results indicate that the translational readthrough is mediated by at least 50 bases long RNA sequence located 3′ to the gag UAG termination codon. Within this sequence a short purine-rich sequence adjacent to the amber codon, highly conserved among different retroviruses, appears essential for M-MuLV suppression. Two alternative putative stem and loop like RNA structures can be drawn at the gag-pol junction, one abutting the gag UAG codon, and the second downstream to it. None of these structures appears to be important to the suppression process. Published by Elsevier Inc. 1991-07 2004-07-22 /pmc/articles/PMC7131665/ /pubmed/2053284 http://dx.doi.org/10.1016/0042-6822(91)90144-Z Text en Copyright © 1991 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Honigman, Alik
Wolf, Dana
Yaish, Shoshanit
Falk, Haya
Panet, Amos
cis acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus
title cis acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus
title_full cis acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus
title_fullStr cis acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus
title_full_unstemmed cis acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus
title_short cis acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus
title_sort cis acting rna sequences control the gag-pol translation readthrough in murine leukemia virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131665/
https://www.ncbi.nlm.nih.gov/pubmed/2053284
http://dx.doi.org/10.1016/0042-6822(91)90144-Z
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