Multiplexed Mass Spectrometry of Individual Ions Improves Measurement of Proteoforms and Their Complexes

A new Orbitrap-based ion analysis procedure is shown to be possible by determining the direct charge for numerous individual protein ions to generate true mass spectra. The deployment of an Orbitrap system for charge detection enables the characterization of highly complicated mixtures of proteoform...

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Detalles Bibliográficos
Autores principales: Kafader, Jared O., Melani, Rafael D., Durbin, Kenneth R., Ikwuagwu, Bon, Early, Bryan P., Fellers, Ryan T., Beu, Steven C., Zabrouskov, Vlad, Makarov, Alexander A., Maze, Joshua T., Shinholt, Deven L., Yip, Ping F., Tullman-Ercek, Danielle, Senko, Michael W., Compton, Philip D., Kelleher, Neil L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131870/
https://www.ncbi.nlm.nih.gov/pubmed/32123391
http://dx.doi.org/10.1038/s41592-020-0764-5
Descripción
Sumario:A new Orbitrap-based ion analysis procedure is shown to be possible by determining the direct charge for numerous individual protein ions to generate true mass spectra. The deployment of an Orbitrap system for charge detection enables the characterization of highly complicated mixtures of proteoforms and their complexes in both denatured and native modes of operation, revealing information not obtainable by traditional measurement of an ensemble of ions.

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