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Anti‐ageing effects of red ginseng on female Drosophila melanogaster

Red ginseng (RG) was recently reported to extend the lifespan of Drosophila melanogaster. However, the mechanism underlying this effect has not yet been elucidated. The present study aimed to elucidate the molecular mechanisms of the RG‐mediated prolongation of the lifespan of female D melanogaster....

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Autores principales: Hou, Wei, Pei, Jin, Wang, YingPing, Zhang, Jiao, Zheng, HouSheng, Cui, Ranji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131930/
https://www.ncbi.nlm.nih.gov/pubmed/32022406
http://dx.doi.org/10.1111/jcmm.15029
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author Hou, Wei
Pei, Jin
Wang, YingPing
Zhang, Jiao
Zheng, HouSheng
Cui, Ranji
author_facet Hou, Wei
Pei, Jin
Wang, YingPing
Zhang, Jiao
Zheng, HouSheng
Cui, Ranji
author_sort Hou, Wei
collection PubMed
description Red ginseng (RG) was recently reported to extend the lifespan of Drosophila melanogaster. However, the mechanism underlying this effect has not yet been elucidated. The present study aimed to elucidate the molecular mechanisms of the RG‐mediated prolongation of the lifespan of female D melanogaster. In this study, protein changes in 36‐day‐old female D melanogaster were identified using isobaric tag for relative and absolute quantitation (iTRAQ), and levels of differentially expressed proteins were verified by quantitative real‐time PCR and Western blotting. Our studies have shown that RG concentrations of 12.5, 15 and 17.5 mg/mL significantly prolonged the lifespan. Eleven proteins were up‐regulated and 46 were down‐regulated between the RG and control groups; and Pebp1 expression was significantly down‐regulated. In addition, AKT and p‐AKT were down‐regulated, and ERK, p‐ERK and Raf1 were up‐regulated by RG. Therefore, RG significantly prolonged the lifespan of female D melanogaster by reducing the expression of Pebp1, up‐regulating ERK and inhibiting the AKT pathway. RG may be a potential drug for anti‐ageing treatment.
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spelling pubmed-71319302020-04-06 Anti‐ageing effects of red ginseng on female Drosophila melanogaster Hou, Wei Pei, Jin Wang, YingPing Zhang, Jiao Zheng, HouSheng Cui, Ranji J Cell Mol Med Short Communication Red ginseng (RG) was recently reported to extend the lifespan of Drosophila melanogaster. However, the mechanism underlying this effect has not yet been elucidated. The present study aimed to elucidate the molecular mechanisms of the RG‐mediated prolongation of the lifespan of female D melanogaster. In this study, protein changes in 36‐day‐old female D melanogaster were identified using isobaric tag for relative and absolute quantitation (iTRAQ), and levels of differentially expressed proteins were verified by quantitative real‐time PCR and Western blotting. Our studies have shown that RG concentrations of 12.5, 15 and 17.5 mg/mL significantly prolonged the lifespan. Eleven proteins were up‐regulated and 46 were down‐regulated between the RG and control groups; and Pebp1 expression was significantly down‐regulated. In addition, AKT and p‐AKT were down‐regulated, and ERK, p‐ERK and Raf1 were up‐regulated by RG. Therefore, RG significantly prolonged the lifespan of female D melanogaster by reducing the expression of Pebp1, up‐regulating ERK and inhibiting the AKT pathway. RG may be a potential drug for anti‐ageing treatment. John Wiley and Sons Inc. 2020-02-05 2020-03 /pmc/articles/PMC7131930/ /pubmed/32022406 http://dx.doi.org/10.1111/jcmm.15029 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Hou, Wei
Pei, Jin
Wang, YingPing
Zhang, Jiao
Zheng, HouSheng
Cui, Ranji
Anti‐ageing effects of red ginseng on female Drosophila melanogaster
title Anti‐ageing effects of red ginseng on female Drosophila melanogaster
title_full Anti‐ageing effects of red ginseng on female Drosophila melanogaster
title_fullStr Anti‐ageing effects of red ginseng on female Drosophila melanogaster
title_full_unstemmed Anti‐ageing effects of red ginseng on female Drosophila melanogaster
title_short Anti‐ageing effects of red ginseng on female Drosophila melanogaster
title_sort anti‐ageing effects of red ginseng on female drosophila melanogaster
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131930/
https://www.ncbi.nlm.nih.gov/pubmed/32022406
http://dx.doi.org/10.1111/jcmm.15029
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