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Open-Source Joystick Manipulandum for Decision-Making, Reaching, and Motor Control Studies in Mice

To make full use of optogenetic and molecular techniques in the study of motor control, rich behavioral paradigms for rodents must rise to the same level of sophistication and applicability. We describe the layout, construction, use and analysis of data from joystick-based reaching in a head-fixed m...

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Detalles Bibliográficos
Autores principales: Belsey, Parley P., Nicholas, Mark A., Yttri, Eric A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Neuroscience 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131984/
https://www.ncbi.nlm.nih.gov/pubmed/32094292
http://dx.doi.org/10.1523/ENEURO.0523-19.2020
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author Belsey, Parley P.
Nicholas, Mark A.
Yttri, Eric A.
author_facet Belsey, Parley P.
Nicholas, Mark A.
Yttri, Eric A.
author_sort Belsey, Parley P.
collection PubMed
description To make full use of optogenetic and molecular techniques in the study of motor control, rich behavioral paradigms for rodents must rise to the same level of sophistication and applicability. We describe the layout, construction, use and analysis of data from joystick-based reaching in a head-fixed mouse. The step-by-step guide is designed for both experienced rodent motor labs and new groups looking to enter into this research space. Using this platform, mice learn to consistently perform large, easily-quantified reaches, including during a two-armed bandit probabilistic learning task. The metrics of performance (reach trajectory, amplitude, speed, duration, and inter-reach interval) can be used to quantify behavior or administer stimulation in closed loop with behavior. We provide a highly customizable, low cost and reproducible open-source behavior training platform for studying motor control, decision-making, and reaching reaction time. The development of this software and hardware platform enables behavioral work to complement recent advances in rodents, while remaining accessible to smaller institutions and labs, thus providing a high-throughput method to study unexplored features of action selection, motivation, and value-based decisions.
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spelling pubmed-71319842020-04-06 Open-Source Joystick Manipulandum for Decision-Making, Reaching, and Motor Control Studies in Mice Belsey, Parley P. Nicholas, Mark A. Yttri, Eric A. eNeuro Open Source Tools and Methods To make full use of optogenetic and molecular techniques in the study of motor control, rich behavioral paradigms for rodents must rise to the same level of sophistication and applicability. We describe the layout, construction, use and analysis of data from joystick-based reaching in a head-fixed mouse. The step-by-step guide is designed for both experienced rodent motor labs and new groups looking to enter into this research space. Using this platform, mice learn to consistently perform large, easily-quantified reaches, including during a two-armed bandit probabilistic learning task. The metrics of performance (reach trajectory, amplitude, speed, duration, and inter-reach interval) can be used to quantify behavior or administer stimulation in closed loop with behavior. We provide a highly customizable, low cost and reproducible open-source behavior training platform for studying motor control, decision-making, and reaching reaction time. The development of this software and hardware platform enables behavioral work to complement recent advances in rodents, while remaining accessible to smaller institutions and labs, thus providing a high-throughput method to study unexplored features of action selection, motivation, and value-based decisions. Society for Neuroscience 2020-03-24 /pmc/articles/PMC7131984/ /pubmed/32094292 http://dx.doi.org/10.1523/ENEURO.0523-19.2020 Text en Copyright © 2020 Belsey et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Open Source Tools and Methods
Belsey, Parley P.
Nicholas, Mark A.
Yttri, Eric A.
Open-Source Joystick Manipulandum for Decision-Making, Reaching, and Motor Control Studies in Mice
title Open-Source Joystick Manipulandum for Decision-Making, Reaching, and Motor Control Studies in Mice
title_full Open-Source Joystick Manipulandum for Decision-Making, Reaching, and Motor Control Studies in Mice
title_fullStr Open-Source Joystick Manipulandum for Decision-Making, Reaching, and Motor Control Studies in Mice
title_full_unstemmed Open-Source Joystick Manipulandum for Decision-Making, Reaching, and Motor Control Studies in Mice
title_short Open-Source Joystick Manipulandum for Decision-Making, Reaching, and Motor Control Studies in Mice
title_sort open-source joystick manipulandum for decision-making, reaching, and motor control studies in mice
topic Open Source Tools and Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131984/
https://www.ncbi.nlm.nih.gov/pubmed/32094292
http://dx.doi.org/10.1523/ENEURO.0523-19.2020
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